Recombinant Human EpCAM/TROP1 Avi-tag His-tag Protein, CF

Catalog #: AVI9277 Datasheet / COA / SDS

Biotinylated

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AVI9277-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EpCAM/TROP1 Avi-tag His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag (Catalog # AVI9277) binds to Human EpCAM/TROP-1 Antibody (Catalog # AF960) with a ED₅₀ of 0.750-7.50 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human EpCAM/TROP1 protein

Human EpCAM (Gln24-Lys265) Accession # P16422.2	Avi-tag	6-His tag
N-terminus		C-terminus

Accession #

P16422.2

N-terminal Sequence
Analysis

Gln24

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

31 kDa

SDS-PAGE

35-42 kDa, under reducing conditions.

Product Datasheets

AVI9277

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9277

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag Protein (Catalog # AVI9277) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-42 kDa.

Reconstitution Calculator

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16). Our Avi-tag Biotinylated Recombinant Human EpCAM features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Strnad, J. et al. (1989) Cancer Res. 49:314.
Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
Ng, V.Y. et al. (2010) Stem Cells 28:29.
Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
de Boer, C.J. et al. (1999) J. Pathol. 188:201.
Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
Schon, M.P. et al. (1993) Int. J. Cancer 55:988.
Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.

Long Name

Epithelial Cell Adhesion Molecule

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Alternate Names

17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A