Recombinant Human Endorepellin Protein, CF Summary
Product Specifications
Arg3684-Ser4391 (Ser4004Thr), with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2364-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human Endorepellin/Perlecan (Catalog # 2364-ER) binds with an ED50 of 0.75-6 µg/mL.
Reconstitution Calculator
Background: Endorepellin/Perlecan
Endorepellin is an 80 kDa glycoprotein that is derived from the C-terminal end of the heparan sulfate proteoglycan, Perlecan. Perlecan itself is an approximately 850 kDa modular protein that contains multiple LDLR, EGF-like, Laminin-like, and immunoglobulin-like domains. Mouse Perlecan lacks several of the Ig-like domains found in the human protein (1-3). Endorepellin corresponds to domain V of Perlecan and consists of three Laminin G domains separated by four EGF-like domains (4). Human Endorepellin shares 89% amino acid sequence identity with mouse Endorepellin. A 26 kDa fragment of Endorepellin is known as LG3 and contains the third Laminin G-like domain of Endorepellin. LG3 can be released by Cathepsin L or BMP-1/Tolloid family mediated cleavage (4-6). Endorepellin binds to Integrin alpha 2/ beta 1, preventing the integrin-dependent adhesion of vascular endothelial cells (EC) to fibronectin and collagen I (7, 8). In contrast, this interaction enhances platelet adhesion to collagen as well as platelet aggregation and activation (9). Endorepellin additionally binds to VEGF R1 and VEGF R2 on EC (10). Its binding to VEGF R2 and Integrin alpha 2/ beta 1 on EC induces the association and down‑regulation of both proteins, followed by the inhibition of EC migration, tubulogenesis, secretion of VEGF, and the activation of multiple receptors involved in angiogenesis (4, 7, 8, 10, 11). Endorepellin also binds to Endostatin, resulting in a reduction of the anti-angiogenic activity of both proteins (4). It preferentially interacts with tumor vasculature and inhibits tumor growth and angiogenesis (8, 12).
- Whitelock, J.M. et al. (2008) Biochemistry 47:11174.
- Murdoch, A.D. et al. (1992) J. Biol. Chem. 267:8544.
- Noonan, D.M. et al. (1991) J. Biol. Chem. 266:22939.
- Mongiat, M. et al. (2003) J. Biol. Chem. 278:4238.
- Cailhier, J.-F. et al. (2008) J. Biol. Chem. 283:27220.
- Gonzalez, E.M. et al. (2005) J. Biol. Chem. 280:7080.
- Bix, G. et al. (2004) J. Cell Biol. 166:97.
- Woodall, B.P. et al. (2008) J. Biol. Chem. 283:2335.
- Bix, G. et al. (2007) Blood 109:3745.
- Goyal, A. et al. (2011) J. Biol. Chem. 286:25947.
- Nystrom, A. et al. (2009) Blood 114:4897.
- Bix, G. et al. (2006) J. Natl. Cancer Inst. 98:1634.
Citations for Recombinant Human Endorepellin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation
Authors: ME Katt, RM Linville, LN Mayo, ZS Xu, PC Searson
Fluids Barriers CNS, 2018-02-20;15(1):7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components
Invest Ophthalmol Vis Sci, 2016-09-01;57(11):4599-605.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Average Rating: 5 (Based on 2 Reviews)
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Reason for Rating: Dissolved easily and provided repeatable, expected results
Experiment was done to study effect of endorepellin on capillary-like tube formation of HUVEC cells
