Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF
Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF Summary
Product Specifications
Met1 - Asp213 (VHL), Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Ala 745 with a N-terminal 10-His tag Sumo tag (CUL2), Met1 - His108 (RBX1)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-655
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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1 μg and 2 μg per lane of Recombinant Human VHL/ELOB/ELOC/CUL2/RBX1 Complex (Catalog # E3-655) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing bands corresponding to the individual proteins of the complex.
Reconstitution Calculator
Background: VHL/ELOB/ELOC/CUL2/RBX1 Complex
VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.
- Kamura T., et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872
- Okumura F., et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010
- Xie L., et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444
- Zhang J., et al. (2018) Science doi: 10.1126/science.aap8411
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