Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF

Catalog #: AVI9298 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI9298-050

Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag (AVI9298) protein is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) with an ED₅₀ of 20‑100 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human DNAM-1/CD226 protein

Human DNAM-1 (Glu19-Asn247) Accession # Q15762.2	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

Q15762.2

N-terminal Sequence
Analysis

Glu19

Structure / Form

Biotinylated protein via Avi-tag

Predicted Molecular Mass

29 kDa

SDS-PAGE

35-60 kDa, under reducing conditions

Product Datasheets

AVI9298

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9298

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag protein (Catalog # AVI9298) is immobilized onto a Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human CD155/PVR Fc Chimera (Catalog # 9147-CD) with an ED₅₀ of 20-100 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human DNAM-1/CD226 His-tag Avi-tag (Catalog # AVI9298) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-60 kDa.

Reconstitution Calculator

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

References

Zingoni, A. et al. (2013) Front. Immunol. 3:408.
Shibuya, A. et al. (1996) Immunity 4:573.
Bottino, C. et al. (2003) J. Exp. Med. 198:557.
Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
Morimoto, K. et al. (2007) Oncogene 27:264.
Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
Shibuya, K. et al. (1999) Immunity 11:615.
Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Long Name

DNAX Accessory Molecule 1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Alternate Names

CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1