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Recombinant Human Dermatopontin Protein, CF

Catalog #: 4629-DP
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4629-DP-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Dermatopontin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to modulate collagen fibrillogenesis. 20 µg/mL of rhDermatopontin can significantly enhance the rate collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived human Dermatopontin protein
Gln19-Val201, with a C-terminal 6-His tag
Accession #
AAH33736
N-terminal Sequence
Analysis
No results obtained: Gln19 predicted
Predicted Molecular Mass
22.8 kDa
SDS-PAGE
23 kDa, reducing conditions

Product Datasheets

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4629-DP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4629-DP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dermatopontin

Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature human Dermatopontin shares 96%, 92%, and 92% amino acid sequence identity with bovine, mouse, and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6-8). Dermatopontin is down-regulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).

References
  1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
  2. Superti-Furga, A. et al. (1993) Genomics 17:463.
  3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
  4. Cronshaw, A.D. et al. (1993) Matrix 13:255.
  5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
  6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
  7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
  8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
  9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
  10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
  11. Okamoto, O. et al. (1996) J. Biochem. 119:106.
  12. Okamoto, O. et al. (1999) Biochem. J. 337:537.
  13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
  14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.
Entrez Gene IDs
1805 (Human); 56429 (Mouse); 289178 (Rat)
Alternate Names
Dermatopontin; DPT; Eq1; TRAMP; Tyrosine-rich acidic matrix protein

Citations for Recombinant Human Dermatopontin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dermatopontin Influences the Development of Obesity-Associated Colon Cancer by Changes in the Expression of Extracellular Matrix Proteins
    Authors: V Catalán, P Domench, J Gómez-Ambr, B Ramírez, S Becerril, A Mentxaka, A Rodríguez, V Valentí, R Moncada, J Baixauli, C Silva, J Escalada, G Frühbeck
    International Journal of Molecular Sciences, 2022-08-17;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  2. Dermatopontin, A Novel Adipokine Promoting Adipose Tissue Extracellular Matrix Remodelling and Inflammation in Obesity
    Authors: X Unamuno, J Gómez-Ambr, B Ramírez, A Rodríguez, S Becerril, V Valentí, R Moncada, C Silva, J Salvador, G Frühbeck, V Catalán
    J Clin Med, 2020-04-09;9(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Role of dermatopontin in re-epithelialization: implications on keratinocyte migration and proliferation.
    Authors: Krishnaswamy, Venkat R, Korrapati, Purna Sa
    Sci Rep, 2014-12-09;4(0):7385.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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