Recombinant Human Dectin-2/CLEC6A Protein, CF Summary
Product Specifications
Thr46-Leu209, with an N-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3114-DC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Dectin-2/CLEC6A
Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4 - 6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).
- Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
- Kanazawa, N. et al. (2004) Immunobiology 209:179.
- Flornes, L.M. et al. (2004) Immunogenetics 56:506.
- Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.
- Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.
- Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
- McGreal, E.P. et al. (2006) Glycobiology 16:422.
- Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
- Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
- Aragane, Y. et al. (2003) J. Immunol. 171:3801.
Citations for Recombinant Human Dectin-2/CLEC6A Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris
Authors: M Bruno, S Kersten, JM Bain, M Jaeger, D Rosati, MD Kruppa, DW Lowman, PJ Rice, B Graves, Z Ma, YN Jiao, A Chowdhary, G Renieris, FL van de Vee, BJ Kullberg, EJ Giamarello, A Hoischen, NAR Gow, AJP Brown, JF Meis, DL Williams, MG Netea
Nat Microbiol, 2020-08-24;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins
Authors: C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge
FASEB J., 2018-01-29;0(0):fj201700619R.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
Authors: KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge
Front Microbiol, 2017-03-07;8(0):321.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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