Recombinant Human DEC-205/CD205 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10205-DE-050
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Recombinant Human DEC-205/CD205 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD302/CLEC13A Fc Chimera (Catalog # 10203-CL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human DEC-205/CD205 Fc Chimera (Catalog # 10205-DE) binds with an ED50 of 4-28 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human DEC-205/CD205 protein
Human DEC-205/CD205
(Ser28-Asp1666)
Accession # O060449.3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
215 kDa
SDS-PAGE
200-250 kDa, under reducing conditions

Product Datasheets

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10205-DE

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10205-DE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant CD302/CLEC13A Fc Chimera (10203-CL) is immobilized at 5 µg/mL, 100 µL/well, Recombinant Human DEC-205/CD205 Fc Chimera (Catalog # 10205-DE) binds with an ED50 of 4-28 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly75), is a type I transmembrane protein that is part of the macrophage mannose receptor (MMR) family of C-type lectins (1). Both the human and mouse proteins share a similar extracellular (ECD) structure consisting of a cysteine rich N-terminal domain, a fibronectin type II domain, and multiple C-type lectin-like domains (1, 2). The mature ECD of human DEC-205 shares 68% amino acid (aa) identity with mouse DEC‑205. DEC‑205 is primarily expressed on dendritic cells (DC) of lymphoid tissues and at lower levels on macrophages and T cells (3, 4). DEC-205 functions as an endocytic receptor for antigens (3, 4). DEC-205 was shown to be a a cell surface receptor for CpG-oligonucleotides (ODN), as mice deficient in DEC-205 have impaired DC and B-cell maturation (5). DEC-205 expressing Kupffer stellate macrophage cells in the liver responded to CpG-ODN and subsequently released cytokines to promote NKT cell activation, leading to liver damage (6). DEC-205 also recognizes apoptotic and necrotic cells by binding to keratin in a pH-dependent fashion, leading to either immune activation or tolerance in the absence or presence of inflammatory stimuli (7, 8). DEC-205 positive macrophages in the human gastric mucosa were shown to mediate local and systemic immune responses to H. pylori infection, which affects systemic immunity (9). DEC-205 was also found to exist as an intergenic splice variant able to form a fusion protein with CD302 in Hodgkin's lymphoma cell lines (10). The DEC-205/CD302 fusion protein was also found to be expressed by mature dendritic cells which altered endocytic capacity of DEC-205, although the wild-type single gene transcripts were the dominant isoforms expressed (11). DEC-205 has been widely used in immune therapies for vaccine generation. Targeting of antigens to DEC-205 resulted in enhanced antigen presentation by DC. In combination with adjuvants, proteins coupled to antibodies against DEC-205 induced strong pathogen-specific immune responses, while without additional adjuvant tolerance could be induced (12).

References
  1. Butler, M. et al. (2007) Immunology. 120:362.
  2. Jiang, W. et al. (1995) Nature. 375:151.
  3. Bonifaz, L. et al. (2002) Jour. Exp. Med. 196:1627.
  4. Shrimpton, R. et al. (2009) Mol. Immunol. 46:1229.
  5. Lahoud MH. et al. (2018) Proc Natl Acad Sci U.S.A. 109:16270.
  6. Hou X. et al. (2017) Cell Mol Immunol. 14:675.
  7. Cao L. et al. (2016) Proc Natl Acad Sci U.S.A. 113:13438.
  8. Hawiger D. et al. (2001) J Exp Med. 194:769.
  9. Kita M. et al. (2018) Oncotarget. 9:15828.
  10. Kato, M. et al. (2003) J Biol Chem. 278:34035.
  11. Butler M. et al. (2017) J. Immunol. 120:362.
  12. Niezold T. et al. (2015) Immunology. 145:519.
Entrez Gene IDs
4065 (Human); 17076 (Mouse)
Alternate Names
CD205; CLEC13B; C-type lectin domain family 13 member B; DEC205; DEC-205; GP200-MR6; Ly75; Ly-75; Lymphocyte antigen 75

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