Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF New

Catalog #: AVI11533 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI11533-050

Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CRACC/SLAMF7 His-tag (Catalog # AVI11533) binds Human CRACC/SLAMF7 Antibody (Catalog # MAB19061) with an ED₅₀ of 5.00-60.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CRACC/SLAMF7 protein
Ser23-Met226, With a C-terminal 6-His tag & Avi-tag

Accession #

Q9NQ25.1

N-terminal Sequence
Analysis

Ser 23

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

25 kDa

SDS-PAGE

44-52 kDa, under reducing conditions

Product Datasheets

AVI11533

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11533

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein (Catalog # AVI11533) binds Human CRACC/SLAMF7 Antibody (MAB19061) with an ED₅₀ of 5.00-60.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein (Catalog # AVI11533) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-52 kDa, under reducing conditions.

Reconstitution Calculator

Background: CRACC/SLAMF7

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CRACC consists of an extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a transmembrane segment, and a cytoplasmic domain with one immunoreceptor tyrosine-based switch motif (ITSM) (2, 3). Within the ECD, human CRACC shares 54% and 52% amino acid sequence identity with mouse and rat CRACC, respectively. There are seven known isoforms of CRACC which are distinguished by deletions and/or substitutions in both their ECD and cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). Its homophilic interaction induces NK, CTL, and B cell activation (4-7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).Our Avi-tag Biotinylated Recombinant Human CRACC/SLAMF7 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Veillette, A. (2006) Immunol. Rev. 214:22.
Tovar, V. et al. (2002) Immunogenetics 54:394.
Murphy, J.J. et al. (2002) Biochem. J. 361:431.
Bouchon, A. et al. (2001) J. Immunol. 167:5517.
Lee, J.K. et al. (2007) J. Immunol. 179:4672.
Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.

Entrez Gene IDs

57823 (Human); 75345 (Mouse); 102133710 (Cynomolgus Monkey)

Alternate Names

19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7