Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein, CF

Catalog #: 10360-AR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10360-AR-050

Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CMG-2/ANTXR2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), it binds Recombinant Human CMG‑2/ANTXR2 Fc Chimera (Catalog # 10360-AR) with an ED₅₀ of 1-6 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CMG-2/ANTXR2 protein

Human CMG-2/ANTXR2 (Gln34-Asn317) Accession # P58335.5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P58335

N-terminal Sequence
Analysis

Gln34 inferred from enzymatic pyroglutamate treatment revealing Glu35

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

57 kDa

SDS-PAGE

55-75 kDa, under reducing conditions

Product Datasheets

10360-AR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10360-AR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), Recombinant Human CMG-2/ANTXR2 Fc Chimera (Catalog # 10360-AR) binds with an ED₅₀ of 1-6 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CMG-2/ANTXR2 Fc Chimera (Catalog # 10360-AR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Reconstitution Calculator

Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1 - 3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1 - 5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.

References

Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106.
Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
Santelli, E. et al. (2004) Nature 430:905.
Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
Abrami, L. et al. (2006) J. Cell Biol. 172:309.
Wei, W. et al. (2006) Cell 124:1141.
Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.

Long Name

Capillary Morphogenesis Protein 2

Entrez Gene IDs

118429 (Human); 71914 (Mouse)

Alternate Names

anthrax toxin receptor 2; ANTXR2; Capillary morphogenesis gene 2 protein; capillary morphogenesis protein 2; cI-35; CMG2; CMG-2; CMG2MGC111533; CMG-2MGC45856; FLJ31074; ISH; JHF; JHS