Recombinant Human CLEC2L Fc Chimera Protein, CF

Catalog #: 10204-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10204-CL-050

Recombinant Human CLEC2L Fc Chimera Protein Binding Activity

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CLEC2L Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin-3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is 1.5-12 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human CLEC2L protein

MD	Human IgG_2A (Pro100-Lys333)	IEGR	Human CLEC2L (Ser90-Thr214) Accession # P0C7M8-1
N-terminus			C-terminus

Accession #

P0C7M8-1

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

39-44 kDa, under reducing conditions

Product Datasheets

10204-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10204-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Galectin-3 (Catalog # 1154-GA) binds with an ED₅₀ of 1.5-12 μg/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-44 kDa and 80-90 kDa, respectively.

Reconstitution Calculator

Background: CLEC2L

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

References