Recombinant Human CLEC14A Fc Chimera Protein, CF

Catalog #: 9664-CL Datasheet / COA / SDS

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9664-CL-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CLEC14A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CLEC14A Fc Chimera is coated at 1 µg/mL, 100 μL/well, Recombinant Human VEGF R3/Flt‑4 Fc Chimera (Catalog # 349-F4) binds with an ED₅₀ of 0.8-4.8 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human CLEC14A protein

Human CLEC14A (Glu22-Ala397) Accession # Q86T13	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q86T13

N-terminal Sequence
Analysis

Glu22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

66 kDa

SDS-PAGE

94-108 kDa, reducing conditions

Product Datasheets

9664-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9664-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CLEC14A

C-type lectin domain family 14 member A (CLEC14A), also known as epidermal growth factor receptor 5 (EGFR-5), is an approximately 52-kDa member of the C-type lectin domain family that plays a role in angiogenic functions including cell-cell adhesion, endothelial cell migration, and tube formation (1, 2). This tumor endothelial marker is a type I transmembrane protein whose extracellular domain (ECD) domain consists of a 141 amino acid (aa) C-type lectin domain, a 43 aa EGF-like domain, and a sushi-like domain (3). Within the ECD, human CLEC14A shares 67% and 68% sequence identity with mouse and rat CLEC14A, respectively.In endothelial cells, CLEC14A forms a complex with VEGFR-3. Failure in the formation of this complex has been observed to result in the enhanced expression and activity of VEGFR-2 and the downstream signaling (4). By its interaction with VEGFR-3, CLECA4 appears to regulate developmental angiogenesis, lymphangiogenesis, and pathological angiogensis (4). CLEC14A acts in vascular homeostasis by fine-tuning VEGF R2 and VEGF R3 signaling in ECs, suggesting its relevance in the pathogenesis of angiogenesis-related human disorders (4).

References

Jang, J. et al. (2017) Sci. Rep. 7:10666.
Mura, M. et al. (2012) Oncogene. 31:293.
Zelensky, A. and Gready, J. (2005) FEBS. J. 272:6179.
Lee, S. et al. (2017) J. Clin. Invest. 127:457.

Long Name

C-type Lectin Domain Family 14, Member A

Entrez Gene IDs

161198 (Human); 66864 (Mouse); 314148 (Rat)

Alternate Names

C14orf27; CEG1; chromosome 14 open reading frame 27; CLEC14A; ClECT and EGF-like domain containing protein; C-type lectin domain family 14 member A; C-type lectin domain family 14, member A; EGF R5; EGFR5; EGFR-5; Epidermal growth factor receptor 5