Recombinant Human CD8 alpha/beta Heterodimer Protein, CF

Catalog #: 9358-CD Datasheet / COA / SDS

Analyzed by SEC-MALS

Catalog #	Availability	Size / Price	Qty
9358-CD-050

Recombinant Human CD8 alpha/beta Heterodimer Protein SEC-MALS.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD8 alpha/beta Heterodimer Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. Bechard, D. et al. (2001) J. Immunol. 167:3099. The ED₅₀ for this effect is 0.8-4 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD8 protein

Human CD8 alpha (Ser22-Asp182) Accession # P01732	Acidic Tail	HHHHHH
Human CD8 beta (Asn19-Pro170) Accession # P10966	Basic Tail	FLAG (DYKDDDDK)
N-terminus		C-terminus

Accession #

P01732 (CD8 alpha ) & P10966 (CD8 beta)

N-terminal Sequence
Analysis

Ser22 (CD8 alpha ) & Asn19 (CD8 beta )

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

26 kDa (CD8 alpha ) & 26 kDa (CD8 beta )

SDS-PAGE

32-43 kDa, reducing conditions

Product Datasheets

9358-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9358-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS

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Recombinant Human CD8 alpha/beta (Catalog # 9358-CD) has a molecular weight (MW) of 60.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Binding Activity

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Immobilized Recombinant Human CD8 alpha/beta Heterodimer (Catalog # 9358-CD) supports the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.8-4 μg/mL.

Reconstitution Calculator

Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4⁺ CD8⁺) thymocytes and mature CD8⁺ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma δ T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8⁺ DC express primarily alpha beta heterodimers, while splenic CD8⁺ DC primarily express alpha alpha homodimers (8). CD8 alpha ⁺ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.

References

Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
Cole, D.K. et al. (2012) Immunology 137:139.
Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Nakayama, K. et al. (1994) Science 263:1131.
Wang, J. and J.R. Klein (1994) Science 265:1860.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Vremec, D. et al. (1992) J. Exp. Med. 176:47.
Lin, T.J. et al. (1998) J. Immunol. 161:6265.
Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
Belz, G.T. et al. (2004) J. Immunol. 172:1996.
Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2