Recombinant Human CD79A Protein, CF

Catalog #: 9685-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9685-CD-050

Recombinant Human CD79A Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD79A Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human CD79A protein
Leu33-Arg143, with a C-terminal 6-His tag

Accession #

P11912-1

N-terminal Sequence
Analysis

Leu33

Predicted Molecular Mass

13 kDa

SDS-PAGE

38-43 kDa, reducing conditions

Product Datasheets

9685-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9685-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human CD79A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 38 - 43 kDa.

Reconstitution Calculator

Background: CD79A

CD79A (also known as Mb-1, Ig alpha and B cell antigen receptor complex-associated protein alpha-chain) is a 30-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79B. Heterodimers of CD79A and CD79B interacts noncovalently with membrane Ig, forming the B cell antigen receptor (BCR) (1). Within this complex, membrane Ig detects antigen while CD79AB initiates signaling (1). CD79A is essential for the differentiation of pre-B cells, and the pre-BCR regulates the surface expression of IL-7R (2). Mature human CD79A is a type I transmembrane glycoprotein. It contains an extracellular region (aa 33-143) with one C2-type Ig-like domain (aa 33-116), and an ITAM-containing cytoplasmic domain (aa 177-205) (3). Within the ECD, human CD79A shares 57% amino acid sequence identity with mouse and rat CD79A.

References

Tseng, J. et al. (1997) Blood, 89:1513.
Kurosaki, T. (2000) Current opinion in immunology, 12:276.
Radaev, Sergei et al. (2010) Structure 18:934.

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat); 722190 (Rhesus Macaque)

Alternate Names

CD79A antigen (immunoglobulin-associated alpha); CD79a antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A; IGAB-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; MB1; MB-1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein