Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI3644-050
Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein Binding Activity.
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Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag (Catalog # AVI3644) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD48/SLAMF2 protein
Human CD48/SLAMF2
(Gln27-Ser220)
Accession # P09326.2
HHHHHHAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gln27 (Blocked, Predicted)
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
25 kDa
SDS-PAGE
43-51 kDa, under reducing conditions

Product Datasheets

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AVI3644

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI3644

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (1039-2B) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag (Catalog # AVI3644) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag (Catalog # AVI3644) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-51 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins (1-3). The human CD48 cDNA encodes a 243 amino acid (aa) precursor that includes a 26 aa signal sequence, a 194 aa mature protein containing two Ig-like C2-type domains, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. It shares 20%-26% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F‑10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8-10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells (8, 12). High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is co-activating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and non-expressing cells can be targets of NK cell or CTL‑mediated lysis (13, 16). Our Avi-tag Biotinylated human CD48 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. McArdel, S.L. et al. (2016) Clin. Immunol. 164:10.
  2. Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
  3. Loertscher, R. and Lavery, P. (2002) Transpl. Immunol. 9:93.
  4. Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
  5. Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
  6. Kiel, M.J. et al. (2005) Cell 121:1109.
  7. Morandi, B. et al. (2005) J. Immunol. 175:3690.
  8. Kato, K. et al. (1992) J. Exp. Med. 176:1241.
  9. Latchman, Y. et al. (1998) J. Immunol. 161:5809.
  10. Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
  11. Messmer, B. et al. (2006) J. Immunol. 176:4646.
  12. Gao, N. et al. (2005) J. Immunol. 174:4113.
  13. Lee, K-M. et al. (2003) J. Immunol. 170:4881.
  14. Lee, K-M. et al. (2006) Blood 107:3181.
  15. McNerney, M.E. et al. (2005) Blood 106:1337.
  16. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
Long Name
SLAM Family Member 2
Entrez Gene IDs
962 (Human); 12506 (Mouse); 245962 (Rat); 102134608 (Cynomolgus Monkey)
Alternate Names
BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2

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