Recombinant Human CD47 His-tag Protein, CF

Name: Recombinant Human CD47 His-tag Protein, CF
Brand: R&D Systems
SKU: 9396-CD
Rating: 5 (1 reviews)

Catalog #: 9396-CD 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9396-CD-100

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Recombinant Human CD47 His-tag Protein Bioactivity

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All CD47 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD47 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha /CD172a Fc Chimera (Catalog # 4546-SA) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human CD47 that produces 50% of the optimal binding response is 0.04-0.2 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CD47 protein
Gln19-Pro139, with a C-terminal 6-His tag

Accession #

Q08722

N-terminal Sequence
Analysis

No results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Leu20

Predicted Molecular Mass

15 kDa

SDS-PAGE

37-45 kDa, reducing conditions

Product Datasheets

9396-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9396-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human SIRPa/CD172a Fc Chimera (Catalog # 4546-SA) is immobilized at 2 µg/mL, Recombinant Human CD47 (Catalog # 9396-CD) binds with an ED₅₀ of 0.04-0.2 µg/mL.

Reconstitution Calculator

Background: CD47

CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Human CD47 is an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34 aa C-terminal cytoplasmic tail (3). Alternate splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail (3). Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47. A portion of the N-terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9-11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin‑mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation (15).

References

Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
Campbell, I.G. et al. (1992) Cancer Res. 52:5416.
Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
Oldenborg, P.-A. et al. (2000) Science 288:2051.
Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
Stefanidakis, M. et al. (2008) Blood 112:1280.
de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
Kukreja, A. et al. (2009) Blood 114:3413.
Wang H. et al. (2007) Blood 109:836.

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen