Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

Catalog #: 10479-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10479-CD-050

Recombinant Human CD300e/LMIR6 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect 0.6-6.0 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD300e/LMIR6 protein

Human CD300e/LMIR6 (Leu18-Leu169) Accession # Q496F6.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q496F6.2

N-terminal Sequence
Analysis

Leu18

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

44 kDa

SDS-PAGE

45-58 kDa and 106-125 kDa (non-reducible dimer), under reducing conditions

Product Datasheets

10479-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10479-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.6-6.0 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CD300e/LMIR6 Fc Chimera Protein (10479-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-58 kDa/106-125 kDa (non-reducible dimer) and 106-125 kDa, respectively.

Reconstitution Calculator

Background: CD300e/LMIR6

CD300e, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35-kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 156 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 11 aa cytoplasmic stub (2). The ECD contains an Ig-like V-type domain with a single disulfide bond and an N-linked glycosylation site. Within the ECD, human CD300e shares 57% sequence identity with mouse and rat CD300e. Its transmembrane segment contains a charged lysine residue which mediates CD300e association with the signaling adaptor molecule DAP12 and enables CD300e to function as an activating receptor (2, 3, 5). The ligand of CD300e is unknown; sphingomyelin has been demonstrated to bind human and mouse CD300e (5). CD300e is highly expressed on intermediate and non-classical monocytes, and myeloid dendritic cells (3, 4). CD300e on monocytes from HIV-infected patients under cART is correlated with markers of disease progression and immune inflammation (6). The cross-linking of CD300e enhances LPS-mediated cytokine production by monocytes, enhancing TNF-alpha production while no enhancement is observed on IL-1 beta, IL-6 and IL-10 (7). Our in-house data indicate that CD300e inhibits T cell activation, including anti-CD3-induced IL-2 and IFN-gamma secretion.

References

Clark, G.J. et al. (2009) Trends Immunol. 30:209.
Chung, D.H. et al. (2003) J. Immunol. 141:6541.
Aguilar, H. et al. (2004) J. Immunol. 173:6703.
Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.
Isobe, M. et al. (2018) J. Biol. Chem. 293:3793.
Vitalle, J. et al. (2017) Front. Immunol. 8:836.
Zenarruzabeitia O. et al. (2016) Sci Rep. 6:32693.

Entrez Gene IDs

342510 (Human); 217306 (Mouse)

Alternate Names

CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2