Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein

Catalog #: 966-6C Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
966-6C-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
966-6C-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED₅₀ for this effect is 0.5‑1.5 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED₅₀ for this effect is 25-100 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human CCL21/6Ckine protein

Human 6Ckine (Ser24 - Pro134) (Pro41 Ser) Accession # NP_002980	GS	Human Fractalkine mucin-like stalk (Phe103 - Gln341) Accession # P78423	KQN	6-His tag
N-terminus				C-terminus

Accession #

NP_002980 (6Ckine) & P78423 (Fractalkine Mucin-lik

N-terminal Sequence
Analysis

Ser24

Predicted Molecular Mass

38.3 kDa

SDS-PAGE

95-105 kDa, reducing conditions

Product Datasheets

966-6C (with carrier)

Product Datasheet

COA

SDS

966-6C/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

966-6C

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

966-6C/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CCL21/6Ckine

Fractalkine (also known as neurotactin and CX₃CL1) is one of two known transmembrane chemokines and is the only member of the CX₃C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX₃CR1 and mediates the tight binding of leukocytes expressing CX₃CR1 independently of G protein activation under flow conditions. In the 6Ckine/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human 6Ckine (CCL21), a ligand for CCR7. On a molar basis, soluble 6Ckine and 6Ckine/mucin-like stalk chimera are essentially equipotent in their ability to chemoattract cultured human lymphocytes.

References

Haskell, C.A. et al. (2000) J. Biol. Chem. 275:34183.

Entrez Gene IDs

6366 (Human); 18829 (Mouse); 298006 (Rat)

Alternate Names

6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4