Recombinant Human Carbonic Anhydrase II Protein, CF
Recombinant Human Carbonic Anhydrase II Protein, CF Summary
Product Specifications
Ser2-Lys260, with C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2184-CA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5
- Recombinant Human Carbonic Anhydrase II/CA2 (rhCA2) (Catalog # 2184-CA)
- Substrate: 4-Nitrophenyl Acetate (4-NPA) (Sigma, Catalog # N8130), 100 mM stock in acetone
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhCA2 to 2 ng/µL in Assay Buffer.
- Dilute Substrate to 2 mM in Assay Buffer.
- In a plate, load 50 µL of 2 ng/µL rhCA2, and start the reaction by adding 50 µL of 2 mM Substrate to wells.
- Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 2 mM Substrate.
- Read absorbance at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).
Per Well:- rhCA2: 0.1 µg
- Substrate: 1 mM
Reconstitution Calculator
Background: Carbonic Anhydrase II/CA2
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Shah, G.N. et al. (2004) Hum. Mutat. 24:272.
Citations for Recombinant Human Carbonic Anhydrase II Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Discovery of novel artemisinin-sulfonamidehybrids as potential carbonicanhydraseIX inhibitors with improved antiproliferative activities
Authors: R An, B Lin, S Zhao, C Cao, Y Wang, X Cheng, Y Liu, M Guo, H Xu, Y Wang, Z Hou, C Guo
Bioorg Chem, 2020-10-08;104(0):104347.
Species: N/A
Sample Types: Reference Standard
Applications: Bioassay -
Design, synthesis and biological evaluation of novel carbohydrate-based sulfonamide derivatives as antitumor agents
Authors: S Hao, X Cheng, X Wang, R An, H Xu, M Guo, C Li, Y Wang, Z Hou, C Guo
Bioorg. Chem., 2020-08-27;104(0):104237.
Species: Human
Sample Types: Peptide
Applications: Bioassay -
Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS
Int J Mol Sci, 2016-11-01;17(11):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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