Recombinant Human C1qTNF14/C1qL1 Protein, CF

Catalog #: 9134-TN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9134-TN-050

Recombinant Human C1qTNF14/C1qL1 Protein Bioactivity

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human C1qTNF14/C1qL1 Protein, CF Summary

Product Specifications

Accession #

O75973

N-terminal Sequence
Analysis

Met

Structure / Form

Noncovalently-linked homotrimer

Predicted Molecular Mass

16 kDa

SDS-PAGE

13 kDa, reducing conditions

Product Datasheets

9134-TN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9134-TN

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution	Reconstitute at 1 mg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Scientific Data

Bioactivity

View Larger

When Recombinant Human C1qTNF14/C1qL1 (Catalog # 9134-TN) is coated at 1 µg/mL (100 µL/well), Recombinant Human BAI3 (Catalog # (Catalog # 9106-BA) binds with a typical ED₅₀ of 0.4-2.4 µg/mL.

Reconstitution Calculator

Background: C1qL1

C1qTNF14 (CTRP14), also known as C1qL1, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF14 contains a collagen-like region and one C1q-like domain and can form disulfide-linked heteromers with C1qTNF11/C1qL4 (3, 4). Within the C1q-like domain, human C1qTNF14 shares 100% aa sequence identity with mouse and rat C1qTNF14. C1qTNF14 is expressed in the inferior olive, hippocampus, and cerebral cortex (5). Similarly to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF11/C1qL4, C1qTNF14 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF14/C1qL1 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7).

References

Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
Ressl, S. et al. (2015) Structure 23:688.
Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
Kakegawa, W. et al. (2015) Neuron 85:316.
Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

Long Name

Complement Component 1, Q Subcomponent-Like 1

Entrez Gene IDs

10882 (Human); 23829 (Mouse); 363686 (Rat)

Alternate Names

C1qL1; C1q-related factor; C1QRF; C1QRFCRFC1QTNF14; C1qTNF14; Complement component 1 Q subcomponent-like 1; complement component 1, q subcomponent-like 1; CTRP14