Recombinant Human C1qR1/CD93 Fc Chimera Protein, CF

Catalog #: 2379-CD Datasheet / COA / SDS

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2379-CD-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human C1qR1/CD93 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce TNF-alpha secretion by THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 6-30 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human C1q R1/CD93 protein

Human C1q R/CD93 (Ala24-Lys580) Accession # Q9NPY3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9NPY3

N-terminal Sequence
Analysis

Ala24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

84.7 kDa (monomer)

SDS-PAGE

110-130 kDa, reducing conditions

Product Datasheets

2379-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2379-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: C1qR1/CD93

C1q R1, also known as CD93 and C1q Rp, is an approximately 125 kDa transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) with one C‑type lectin domain, four tandem EGF‑like domains, and a mucin‑like domain, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. It is distinct from the 60 kDa Calreticulin which is likewise known as C1q R1 (4, 5). Unlike Calreticulin, and despite its name, C1q R1/CD93 does not appear to bind the complement protein C1q (3, 5). CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). It is required for plasma cell longevity in the bone marrow and for the resulting extended humoral response (9).

References

Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
Nepomuceno, R.R. et al. (1997) Immunity 6:119.
Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.

Long Name

Complement Component C1q Receptor

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Alternate Names

AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4