Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Protein, CF

Catalog #: 8918-BT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8918-BT-050

Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Protein Bioactivity

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All BTN2A2/Butyrophilin 2A2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.1-0.5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human BTN2A2/Butyrophilin 2A2 protein

Human BTN2A2 (Gln33-Val237) Accession # Q8WVV5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8WVV5

N-terminal Sequence
Analysis

No result obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Phe34

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

Product Datasheets

8918-BT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8918-BT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Chimera (Catalog # 8918-BT) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.1-0.5 μg/mL.

Reconstitution Calculator

Background: BTN2A2/Butyrophilin 2A2

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature human Butyrophilin 2A2 consisits of a 233 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 237 aa cytoplasmic domain. Within the Ig-like domains, human Butyrophilin 2A2 shares 70% aa sequence identity with mouse and rat Butyrophilin 2A2. Alternative splicing generates additional isoforms of human Butyrophilin 2A2 that lack the first, second, or both Ig-like domains as well as isoforms with substitutions and deletions in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3⁺ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

References