Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI10909-050
Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag Protein SDS-PAGE.
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Recombinant Human BTN2A1/Butyrophilin 2A1 Fc Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD277/BTN3A1 Fc Chimera Protein (Catalog # 8539-BT) is immobilized at 10.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag (Catalog #AVI10909) that produces 50% of the binding response is 1.00-6.00 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BTN2A1 protein
Human BTN2A1
(Gln29-Ala248)
Accession # Q7KYR7.3
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Protein identify confirmed by mass spectromety.
Structure / Form
Disulfide-linked homodimer. Biotinylated via Avi-tag.
Predicted Molecular Mass
53 kDa
SDS-PAGE
65-80 kDa, under reducing conditions.

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AVI10909

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10909

Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human BTN2A1 Fc Chimera Avi-tag Protein (Catalog # AVI10909) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa to 130-160 kDa, respectively.

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Background: BTN2A1

Butyrophilin 2A1 (BTN2A1) is a type I transmembrane glycoprotein of the butyrophilin family within the Ig-superfamily. There are over 13 Butyrophilin and the closely related butyrophilin-like (BTNL) molecules and they have been identified as immune checkpoint receptors involved in modulating T cell function (1,2). Mature human BTN2A1 consists of an extracellular domain (ECD) with two immunoglobulin-like domains (one IgV and one IgC), a transmembrane segment, and a cytoplasmic region with a B30.2 domain (3). Alternative splicing generates additional isoforms of BTN2A1 that lack the first Ig-like domain or the transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (4, 5). BTN2A1 binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (4). A polymorphism of BTN2A1 has been associated with metabolic syndromes, type II diabetes mellitus, chronic kidney disease, and hypertension (6, 7). Our Avi-tag Biotinylated BTN2A1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Afrache, H. et al. (2012) Immunogenetics 64:781.
  3. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
  4. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
  5. Cavaletto, M. et al. (2002) Proteomics 2:850.
  6. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
  7. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.
Long Name
Butyrophilin Subfamily 2 Member A1
Entrez Gene IDs
11120 (Human)
Alternate Names
BK14H9.1; BT2.1; BTF1; BTF1BT2.1butyrophilin BTF1; BTN2A1; butyrophilin subfamily 2 member A1; butyrophilin, subfamily 2, member A1; DJ3E1.1; FLJ36567

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