Recombinant Human BTN1A1/Butyrophilin His Avi Protein, CF

Catalog #: AVI8467 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI8467-050

Recombinant Human BTN1A1/Butyrophilin His Avi Protein Binding Activity

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All BTN1A1/Butyrophilin Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BTN1A1/Butyrophilin His Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Accession #

Q13410

N-terminal Sequence
Analysis

Ala27

Structure / Form

Biotinylated protein via Avi-tag

Predicted Molecular Mass

27 kDa

SDS-PAGE

30-43 kDa, under reducing conditions

Product Datasheets

AVI8467

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8467

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Scientific Data

Binding Activity

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When Rabbit Anti-Human BTN1A1/Butyrophilin Monoclonal Antibody (Catalog # MAB84671) is immobilized at 10 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human BTN1A1/Butyrophilin His-tag Avi-tag (Catalog # AVI8467) with an ED₅₀ of 0.01-0.1 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human BTN1A1/Buyrophilin His-tag Avi-tag (Catalog # AvI8467)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 30-43 kDa.

Reconstitution Calculator

Background: BTN1A1/Butyrophilin

Butyrophilin 1A1 (also called BTN1A1), a 55 kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 494 amino acids (aa) long and is composed of an extracellular domain (ECD) (aa 27-242), a transmembrane domain and a cytoplasmic tail (aa 270-526) which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma δ T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4⁺ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of human BTN1A1 shares 68% aa sequence identity with both mouse and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.

References