Recombinant Human BAFFR/TNFRSF13C Fc Chimera Protein, CF

Catalog #: 1162-BR
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1162-BR-050

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Citations (7)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BAFFR/TNFRSF13C Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED₅₀ for this effect is 20-80 ng/mL in the presence of 5 ng/mL recombinant human BAFF.

Source

Mouse myeloma cell line, NS0-derived human BAFF R/TNFRSF13C protein

Human BAFF R (Ser7-Ala71) Accession # Q96RJ3	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q96RJ3

N-terminal Sequence
Analysis

Starts at Ser7

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

33 kDa (monomer)

SDS-PAGE

40-50 kDa, reducing conditions

Product Datasheets

1162-BR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1162-BR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BAFFR/TNFRSF13C

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4⁺ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.

References

Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
Laabi, Y. et al. (2001) Current Biol. 11:R1013.
Thompson, J.S. et al. (2001) Science 14:2108.

Long Name

B cell Activating Factor Receptor

Entrez Gene IDs

115650 (Human); 72049 (Mouse)

Alternate Names

BAFF R; BAFFR; BAFFR;BAFF-R;BROMIX;CD268;CVID4;prolixin;Tumor necrosis factor receptor superfamily member 13C; BR3; CD268; TNFRSF13C

Related Research Areas

Citations for Recombinant Human BAFFR/TNFRSF13C Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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IL-10 producing regulatory B cells are decreased in blood from smokers and COPD patients
Authors: M Jacobs, S Verschraeg, B Salhi, J Anckaert, P Mestdagh, GG Brusselle, KR Bracke
Respiratory Research, 2022-10-17;23(1):287.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Suppressed humoral immunity is associated with dengue nonstructural protein NS1-elicited anti-death receptor antibody fractions in mice
Authors: CL Tsai, DS Sun, MT Su, TS Lien, YH Chen, CY Lin, CH Huang, CC King, CR Li, TH Chen, YH Chiu, CC Lu, HH Chang
Sci Rep, 2020-04-14;10(1):6294.
Species: Human, Rabbit
Sample Types: Protein, Serum
Applications: ELISA Capture
The effects of BAFF on T lymphocytes in chronic obstructive pulmonary disease
Authors: S Gao, J Chen, J Xie, J Wang
Respir. Res., 2020-03-11;21(1):66.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF?&kappaB signaling pathway in glomerular mesangial cells
Authors: Y Cao, G Lu, X Chen, X Chen, N Guo, W Li
Mol Med Rep, 2019-12-06;21(2):795-805.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
BAFF promotes proliferation of human mesangial cells through interaction with BAFF-R.
Authors: Zheng N, Wang D, Ming H, Zhang H, Yu X
BMC Nephrol, 2015-05-15;16(0):72.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
Nat Immunol, 2014-02-23;15(4):354-64.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.
Authors: Nishio M, Endo T, Tsukada N, Ohata J, Kitada S, Reed JC, Zvaifler NJ, Kipps TJ
Blood, 2005-04-28;106(3):1012-20.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay