Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein, CF

Catalog #: 9370-AS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9370-AS-050

Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ASGR2/ASGPR2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ASGR2 Fc Chimer binds human plasma von Willebrand Factor with an ED₅₀ of 0.15-0.9 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human ASGR2 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human ASGR2/ASGPR2 (Gln80-Ala311) Accession # NP_001172
N-terminus			C-terminus

Accession #

NP_001172

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

53 kDa

SDS-PAGE

60-74 kDa, reducing conditions

Product Datasheets

9370-AS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9370-AS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Immobilized Recombinant Human ASGR2 Fc Chimera binds human plasma von Willebrand Factor with an ED₅₀ of 0.15-0.9 μg/mL.

Reconstitution Calculator

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR-2), also known as C-type lectin domain family 4 member H2 (CLEC4H2) and hepatic lectin H2 (HL-2), is an approximately 48 kDa member of the C-type lectin receptor family (1, 2). ASGR-2 is a single-pass type II transmembrane protein that plays a role in the endocytosis of desialylated glycoproteins and hepatic thrombopoietin production (3). ASGR-2 is closely related to ASGR-1 which is about 2 kDa smaller and is encoded by distinct but closely linked genes. Variations in ASGR-2 structure due to alternative splicing have been reported (4-6). Mature ASGR-2 consists of a 5 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment and a 232 aa extracellular domain (ECD). Within the ECD, human ASGR-2 shares 66% and 62% aa sequence identity with mouse and rat ASGR-2, respectively. Both ASGR-2 and ASGR-1 compose the asialoglycoprotein receptor (ASGPR) which mediates removal of potentially hazardous glycoconjugates from blood in health and disease (7). ASGPR, also known as the Ashwell receptor, can modulate von Willebrand factor (vWF) and platelet homeostasis in part through clearance of platelets that are first desialylated during sepsis caused by pathogens including S. pneumoniae. The ASGR-1 chain of the Ashwell receptor can also participate in plasma vWF clearance independently of sialylation and sepsis, ASGR-2 colocalization with plasma vWF was increased in ASGR-1 deficient mice (8). In addition, ASGR-2 expression was shown to be associated with malignant phenotypes in gastric cancer and may represent a specific biomarker for recurrence of some gastric cancers (9).

References

Spiess, M. and H.F. Lodish. (1985) Proc Natl Acad Sci U.S.A. 19: 6465.
Grewal, P.K. (2010) Methods Enzymol. 479:223.
Grozovsky, R. et al. (2015) Nat Med. 1:47.
Drickamer, K. et al. (1984) J. Biol. Chem. 259:770.
Halberg, D.F. et al. (1987) J. Biol. Chem. 262:9828.
Paietta, E. et al. (1992) J. Biol. Chem. 267:11078.
Harris R.L. et al. (2012) Mol Biol Int. 2012:283974.
Grewal, P. et al. (2008) Nature Medicine. 14:6.
Tanaka H. et al. (2018) Mol Cancer Res. doi: 10.1158/1541. MCR-17-0467. [Epub ahead of print].

Long Name

Asialoglycoprotein Receptor 2

Entrez Gene IDs

433 (Human); 11890 (Mouse); 29403 (Rat)

Alternate Names

ASGPR 2; ASGP-R 2; ASGPR2; ASGP-R2; ASGR2; asialoglycoprotein receptor 2; CLEC4H2; CLEC4H2FLJ60040; C-type lectin domain family 4 member H2; HBxAg-binding protein; HBXBP; Hepatic lectin H2; HL-2