Recombinant Human 15-PGDH/HPGD Protein, CF Summary
Product Specifications
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5660-DH
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: 15-PGDH/HPGD
HPGD, or 15-hydroxyprostaglandin dehydrogenase, is a NAD+-linked dehydrogenase that oxidizes the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in a loss of biological activity (1). HPGD is a major enzyme for the catabolism of prostaglandins. The enzyme is a member of the short-chain alcohol dehydrogenase family of enzymes (2). HPGD is a cytosolic enzyme expressed in most tissues, with highest expression levels in placenta, lung, and kidney (3). It is inhibited by aspirin and nonsteroidal anti-inflammatory drugs (4). Defects in HPGD are a cause of hypertrophic osteoarthropathy (5).
- Anggard, E. and B. Samuelsson (1964) J. Biol. Chem. 239:4097.
- Krook, M. et al. (1990) Biochemistry 29:738.
- Tai, H.H. (1976) Biochemistry 15:4586.
- Mak, O.T. et al. (1982) Biosci. Rep. 2:503.
- Uppal, S. et al. (2008) Nat. Genet. 40:789.
FAQs
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Why am I observing an additional higher molecular weight band?
We expect this higher molecular weight band to be the homodimer. In this homodimer, the two monomers are noncovalently associated with each other. Since they are not joined by disulfide bonds, reducing conditions are not expected to separate the two monomers.
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