Home / IL-1 beta/IL-1F2 / Recombinant Equine IL-1 beta/IL-1F2 Protein

Recombinant Equine IL-1 beta/IL-1F2 Protein

Catalog #: 3340-EL
12 Citations 3 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3340-EL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3340-EL-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (12)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Equine IL-1 beta/IL-1F2 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 5-30 pg/mL.
Source
E. coli-derived equine IL-1 beta/IL-1F2 protein
Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys and Arg256Gln)
Accession #
Q28386
N-terminal Sequence
Analysis
Ala116
Predicted Molecular Mass
17 kDa

Product Datasheets

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3340-EL (with carrier)

Product Datasheet
COA
SDS

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3340-EL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3340-EL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3340-EL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5 - 7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65% - 75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.

References
  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
  6. Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  8. Kato, H. et al. (1996) Gene 177:11.
Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Citations for Recombinant Equine IL-1 beta/IL-1F2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Culture media supplemented with 10% equine serum provided chondroprotection in an in vitro co-culture of cartilage and synovial membrane
    Authors: Alvarez, AV;Wooldridge, AA;Fuller, J;Shrader, SM;Mansour, M;Boone, LH;
    Journal of equine veterinary science
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells
    Authors: NA Moyo, D Westcott, R Simmonds, F Steinbach
    Viruses, 2023-01-16;15(1):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Use of allogeneic freeze-dried conditioned serum for the prevention of degradation in cartilage exposed to IL-1�
    Authors: LC Garbin, CW McIlwraith, DD Frisbie
    Oncogene, 2022-07-11;18(1):265.
    Species: Equine
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines
    Authors: B Jeremiasse, C Matta, CR Fellows, DJ Boocock, JR Smith, S Liddell, F Lafeber, WE van Spil, A Mobasheri
    BMC Mol Cell Biol, 2020-06-26;21(1):47.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mass spectrometric analysis of the in vitro secretome from equine bone marrow-derived mesenchymal stromal cells to assess the effect of chondrogenic differentiation on response to interleukin-1&beta treatment
    Authors: L Bundgaard, A Stensballe, KJ Elbæk, LC Berg
    Stem Cell Res Ther, 2020-05-20;11(1):187.
    Species: Horse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. An Objective Assessment of the Effect of Anesthetizing the Median Nerve on Lameness Caused by Pain in the Cubital Joint
    Authors: L McGlinchey, G Agne, T Passler, R Cole, J Schumacher
    J. Equine Vet. Sci., 2018-12-19;75(0):9-13.
    Species: Equine
    Sample Types: In Vivo
    Applications: In Vivo
  7. Inflammatory licensed equine MSCs are chondroprotective and exhibit enhanced immunomodulation in an inflammatory environment
    Authors: JM Cassano, LV Schnabel, MB Goodale, LA Fortier
    Stem Cell Res Ther, 2018-04-03;9(1):82.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Effects of stanozolol on normal and IL-1?-stimulated equine chondrocytes in vitro
    Authors: M Castro Mar, MJ Peffers, K Lee, LM Rubio-Mart
    BMC Vet. Res., 2018-03-20;14(1):103.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10
    Authors: H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach
    Virology, 2018-03-02;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages
    Authors: LJ Berghaus, S Giguère, AI Bordin, ND Cohen
    Cytokine, 2017-12-12;102(0):7-11.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFN?-Primed Equine Mesenchymal Stem Cells
    Authors: Danièle Noel
    Front Immunol, 2016-09-27;7(0):392.
    Species: Equine
    Sample Types: Cartilage Explants
    Applications: Bioassay
  12. Effects of high mobility group box protein-1, interleukin-1beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures.
    Authors: Ley C, Svala E, Nilton A, Lindahl A, Eloranta ML, Ekman S, Skioldebrand E
    Connect. Tissue Res., 2010-11-30;52(4):290-300.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Does IL-1 beta induce NO in smooth muscle?

    • Yes it does, based on the literature.

View all Proteins and Enzyme FAQs

Reviews for Recombinant Equine IL-1 beta/IL-1F2 Protein

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Equine IL-1 beta/IL-1F2 Protein
By Aileen Rowland on 10/26/2017
Application: In vitro bioactivity in cell culture
Reason for Rating: Very easy to follow instructions, worked very well
Recombinant Equine IL-1 beta/IL-1F2 Protein
By Hsing Fann on 09/08/2017
Application: In vitro bioactivity in cell culture
In vitro bioactivity in cell culture Recombinant Equine IL-1 beta/IL-1F2 Protein 3340-EL
Recombinant Equine IL-1 beta/IL-1F2 Protein
By Anonymous on 12/15/2016
Application: Stem/Immune cell maintenance or differentiation

We are testing the effect of inflammatory culture environments on mesenchymal stem cell secretomes to investigate the potential role of MSC in treatment of osteoarthritis.