Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF

Catalog #: 9695-B1 Datasheet / COA / SDS

Fc Chimera

Catalog #	Availability	Size / Price	Qty
9695-B1-100

Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 0.75-4.5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived B7-1/CD80 protein

Cynomolgus Monkey B7-1/CD80 (Val35-Asn242) Accession # XP_005548122	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005548122

N-terminal Sequence
Analysis

Val35

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa

SDS-PAGE

53-79 kDa, reducing conditions

Product Datasheets

9695-B1

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9695-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey B7‑1/CD80 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-79 kDa and 105-160 kDa, respectively.

Reconstitution Calculator

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100-fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-5). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2). Mature cynomolgus monkey B7-1 consists of a 213 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane domain, and a 20 aa cytoplasmic domain. Within the ECD, cynomolgus monkey B7-1 shares 97% and 51% aa sequence identity with human and mouse B7-1, respectively. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas (6).

References

Ville, S. et al. (2015) Front. Immunol. 6:411.
Azuma, M. et al. (1993) Nature 366:76.
Freeman, G.J. et al. (1993) Science 262:909.
Chen, C. et al. (1994) J. Immunol. 152:4929.
Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Mir, M.A., et al. (2008) Expert Opin. Ther. Targets 12:969.

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Alternate Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80