Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

Catalog #: 10482-R2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10482-R2-100

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-21 Protein (Catalog # 8879-IL) is immoblized at 2.5 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (Catalog # 10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived IL-21R protein

Cynomolgus Monkey/Rhesus Macaque IL‑21R (Cys20-Pro236) Accession # XP_005591578.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005591578.1

N-terminal Sequence
Analysis

Cys20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Product Datasheets

10482-R2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10482-R2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-21 Protein (8879-IL) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera Protein (Catalog # 10482-R2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 110-160 kDa, respectively.

Reconstitution Calculator

Background: IL-21R

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma chain ( gamma _c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Based on its similarity with human IL-21 R, mature cynomolgus/rhesus IL-21 R is predicted to consist of a 213 amino acid (aa) extracellular domain (ECD), a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, cynomolgus/rhesus IL-21 R shares 98% aa identity with human IL-21 R. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8⁺ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).

References

Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
Asao, H. et al. (2001) J. Immunol. 167:1.
Habib, T. et al. (2002) Biochemistry 41:8725.
Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Ozaki, K. et al. (2002) Science 298:1630.
Rankin, A.L. et al. (2011) J. Immunol. 186:667.
King, I.L. et al. (2010) J. Immunol. 185:6138.
Kasaian, M.T. et al. (2002) Immunity 16:559.
Frohlich, A. et al. (2009 Science 324:1576.
Tortola, L. et al. (2010) Blood 116:5200.
Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
Moser, E.K. et al. (2015) PLoS One 10:e0120169.
Jin, H. et al. (2009) J. Clin. Invest. 119:47.
Sarosiek, K.A. et al. (2010) Blood 115:570.

Long Name

Interleukin 21 Receptor

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Alternate Names

CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor