Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Catalog #: 10948-RS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10948-RS-025

Recombinant Cynomolgus Monkey R‑Spondin 3 Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.4-10 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey R-Spondin 3 protein
Met33-His273

Accession #

XP_005551811.1

N-terminal Sequence
Analysis

Met33

Predicted Molecular Mass

27 kDa

SDS-PAGE

34-44 kDa, under reducing conditions.

Product Datasheets

10948-RS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10948-RS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Cynomolgus Monkey R-Spondin 3 (Catalog # 10948-RS) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.4‑10 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey R-Spondin 3 Protein (Catalog # 10948-RS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-44 kDa.

Reconstitution Calculator

Background: R-Spondin 3

Roof plate-specific spondin 3 (RSPO3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin1), is member of the R-Spondin family of proteins which are involved in the activation and regulation of Wnt signaling pathway. The R-Spondin family, consisting of 4 members, are extracellular glycoproteins which share ~60% amino acid (aa) homology and a conserved protein structure (1). Similar to other R-Spondins, RSPO3 contains two adjacent cysteine-rich furin-like domains with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif and a C-terminal tail rich in basic residues. The furin-like domains are needed for beta -catenin stabilization (2, 3). Cynomologus RSPO3 shares 97% and 88% aa sequence identity with human and mouse RSPO3, respectively. While every member of the R-Spondin family positive modulators of Wnt/ beta -catenin signaling, each one has a distinct expression pattern (1 - 5). In mouse, RSPO3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (5, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (10). RSPO3 has also been identified as an oncogene (11).

References

Kim, K.-A. et al. (2006) Cell Cycle, 5:23.
Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287.
Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
Kazanskaya, O. et al. (2008) Development 135:3655.
Aoki, M. et al. (2007) Dev. Biol. 301:218.
Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
Theodorou, V. et al. (2007) Nat. Genet. 6:759.

Long Name

Roof Plate-specific Spondin 3

Entrez Gene IDs

84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)

Alternate Names

Cristin 1; CRISTIN1; FLJ14440; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; Roof plate-specific spondin-3; RSPO3; R-spondin 3 homolog (Xenopus laevis); RSpondin 3; R-Spondin 3; R-spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2; THSD2