Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein, CF

Catalog #: 10718-PV Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10718-PV-050

Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey PVRIG Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey PVRIG Fc Chimera is immobilized at 1.00 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds with an ED₅₀ of 0.150-0.750 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey PVRIG protein

Cynomolgus Monkey PVRIG (Gly20, Ser21 or Glu23-Leu172) Accession # XP_005549281.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005549281.1

N-terminal Sequence
Analysis

Gly20, Ser21, Glu23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa

SDS-PAGE

52-63 kDa, under reducing conditions

Product Datasheets

10718-PV

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10718-PV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus Monkey PVRIG Fc Chimera (Catalog # 10718-CV) is immobilized at 1.00 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 (2229-N2) binds with an ED₅₀ of 0.150-0.750 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey PVRIG Fc Chimera (Catalog # 10718-PV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-63 kDa and 104-126 kda, respectively.

Reconstitution Calculator

Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of cynomolgus monkey PVRIG have approximately 90% similarity with human PVRIG, respectively. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1-3). Blocking PVRIG with neutralizing antibody is a feasible approach for NK cell mediated immunotherapy of cancers (4, 5).

References

Zhu, Y. et al. (2016) J. Exp. Med. 213:167.
Whelan, S. et al. (2019) Cancer Immunol Res. 7:257.
Murter, B. et al. (2019) Cancer Immunol Res. 7:244.
Li, J. et al. (2020) Haematologica. DOI:10.3324/haematol.2020.258574.
Sanchez-Correa, B. et al. (2019) Cancers (Basel) 11:877.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Alternate Names

C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; poliovirus receptor related immunoglobulin domain containing; Poliovirus receptor-related immunoglobulin domain-containing protein; PVRIG; transmembrane protein PVRIG