Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

Catalog #: 10485-N2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10485-N2-100

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein Binding Activity

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Nectin‑2/CD112 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (Catalog # 9317-N2) binds with an ED₅₀ of 0.1-0.6 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Nectin-2/CD112 protein

Cynomolgus Monkey (Gln32-Gly360) Accession # XP_005589607.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005589607.1

N-terminal Sequence
Analysis

No results obtained: Gln32 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

62 kDa

SDS-PAGE

68-80 kDa, under reducing conditions

Product Datasheets

10485-N2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10485-N2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Chimera (10485-N2) is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (9276-DN) binds with an ED₅₀ of 0.1-0.6 μg/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Chimera Protein (Catalog # 10485-N2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-80 kDa and 130-160 kDa, respectively.

Reconstitution Calculator

Background: Nectin-2/CD112

Nectins are a small family of Ca⁺⁺-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. Based on its similarity with human Nectin-2, cynomolgus Nectin-2/CD112 (Nectin-2δ) is predicted to consist of a 329 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2). Within the ECD, cynomolgus Nectin-2 shares 96% aa sequence identity with human Nectin-2. Alternative splicing of human Nectin-2 generates a short 60 kDa isoform with a 94 aa cytoplasmic tail (2). Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts (1, 3). It forms homodimers in cis, followed by dimers in trans (between cells) (3). It does not cis-dimerize with other Nectins but forms cis-dimers between its two splice forms. Notably, a Nectin-2 cis-dimer on one cell can heterodimerize with a Nectin-3 cis-dimer on a neighboring cell (3). Nectin-2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity (4, 5). Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus (3, 6). Nectin-2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload (7).

References

Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
Eberle, F. et al. (1995) Gene 159:267.
Struyf, F. et al. (2002) J. Virol. 76:12940.
Bottino, C. et al. (2003) J. Exp. Med. 198:557.
Pende, D. et al. (2005) Mol. Immunol. 42:463.
Warner, M.S. et al. (1998) Virology 246:179.
Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.

Long Name

Poliovirus Receptor Related 2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Alternate Names

CD112 antigen; CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; HVEB; HVEBpoliovirus receptor-like 2; MPH; nectin 2; Nectin2; Nectin-2; poliovirus receptor-related 2 (herpesvirus entry mediator B); poliovirus receptor-related protein 2; PRR2; PRR2nectin-2; PVRL2; PVRR2poliovirus receptor related 2