Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF

Catalog #: 11205-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11205-CD-050

Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein SDS-PAGE.

1 Image

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD-43 Fc Chimera (Catalog # 11205-CD) is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human Siglec-1 Protein (Catalog # 5197-SL) binds with an ED50 of 1.50-7.50 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD43 protein

Cynomolgus Monkey CD43 (Asp20-Thr258) Accession # EHH60309.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

EHH60309.1

N-terminal Sequence
Analysis

Asp20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

SDS-PAGE

106-118 kDa, under reducing conditions.

Product Datasheets

11205-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11205-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein (Catalog # 11205-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 210-240 kDa, respectively.

Reconstitution Calculator

Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis and can either promote or block cell adhesion (3). CD43 with altered glycosylations are expressed in cancers (4). The extracellular portion of cynomolgus monkey CD43 has a 78.2%, and 93.0% identity to human and rhesus monkeys, respectively.. CD43 induced cellular adhesion through the binding to molecules such as E-selectin (5, 6), galectin-1 and galectin-3 (7), siglec-1 (8), M-ficolin (9), integrins (10), cell surface nucleolin (11), and ICAM-1 (intercellular adhesion molecule type 1) (12). During cancer development, CD43 signalling induces the activation of 𝛽-catenin, NF-𝜅B (13, 14), NFAT, and AP-1, which are prosurvival transcription factors that can promote tumorigenesis when deregulated (15, 16).

References

Santamaría, M. et al. (1996) Cancer Res. 56:3526.
Manjunath, N. et al. (1995) Nature 377:535.
Brown, T.J. et al. (1996) J. Biol. Chem. 271:27686.
Tuccillo, F.M. et al. (2014) BioMed Res International. https://doi.org/10.1155/2014/742831.
Fuhlbrigge, R.C. et al. (2006) Blood 107:1421.
Matsumoto, M. et al. (2005) J. Immuno. 175:8042.
Yang, R.Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
van den Berg, T.K. et al. (2001) J. Immuno. 166:3637.
Moreno-Amaral, A.N. et al. (2012) J. Leukoc. Bio. 91:469.
Sanchez-Mateos, P. et al. (1995) Blood, 86:2228.
Hirano, K. et al. (2005) J. Biol. Chem. 280:39284.
Rosenstein, Y. et al. (1991) Nature 354:233.
Fiume, G. et al. (2013) PLoS ONE 8:e66087.
Fiume, G. et al. (2012) Nucleic Acids Res. 40:3548.
Andersson, C.C. et al. (2005) Biochem. J. 387:377.
Santana, M.A. et al. (2000) J Biol. Chem. 275:31460.

Long Name

Cluster of Differentiation 43

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Alternate Names

CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN