Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

Catalog #: 9660-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9660-CD-100

Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera is coated at 1 μg/mL, 100 μL/well, it binds to Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) with an ED₅₀ of 5-25 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD40/TNFRSF5 protein

Cynomolgus Monkey CD40/TNFRSF5 (Glu21-Arg193) Accession # XP_005569274.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005569274.1

N-terminal Sequence
Analysis

Glu21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa

SDS-PAGE

57-64 kDa, reducing conditions

Product Datasheets

9660-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9660-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera (Catalog # 9660-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) binds with an ED₅₀ of 5-25 ng/mL.

Reconstitution Calculator

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature Cynomolgus Monkey CD40 consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain (2). The extracellular domain (21-193 aa) of Cynomolgus Monkey CD40 shares 96%, 58% and 56% amino acid (aa) sequence identity with human, mouse and rat CD40, respectively. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells (6). CD40 ligation by CD40L promotes B cell activation and T cell‑dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40L expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
Eshel, D. et al. (2008) Mol. Immunol. 46:250.
van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
Pamukcu, B. et al. (2011) Ann. Med. 43:331.
Hassan, G.S. et al. (2012) Immunobiology 217:521.

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Alternate Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5