Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein, CF

Catalog #: 9297-DC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9297-DC-100

Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED₅₀ for this effect is 15‑75 ng/mL in the presence of 20 ng/mL Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL).

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DcR3/TNFRSF6B protein

Cynomolgus Monkey DcR3/TNFRSF6B (Ala30-His300) Accession # EHH65162	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

EHH65162

N-terminal Sequence
Analysis

Ala30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

56 kDa

SDS-PAGE

58-67 kDa, reducing conditions

Product Datasheets

9297-DC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9297-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Cynomolgus Monkey DcR3/TNFRSF6B Fc Chimera (Catalog # 9297-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 15-75 ng/mL.

Reconstitution Calculator

Background: DcR3/TNFRSF6B

DcR3 (Decoy Receptor 3), also known as TR6 and TNFRSF6B is a 35-40 kDa secreted member of the TNF Receptor superfamily (1). Mature cynomolgus DcR3 contains four tandem TNFR cysteine-rich domains and shares 92% amino acid sequence identity with human DcR3. It binds to the TNF superfamily ligands Fas Ligand, TL1A, and LIGHT (2-5) and interferes with their respective interactions with Fas, DR3, or HVEM and Lymphotoxin beta R (2-4). It blocks apoptosis triggered through either Fas Ligand, TL1A, or LIGHT (2, 3, 5, 6). DcR3 is up-regulated in a variety of cancers and enhances tumor cell immune evasion (2, 3, 7). It also promotes immune suppression by inducing dendritic cell apoptosis (8), inhibiting NK cell and CD8⁺ T cell activity (2, 4), and inhibiting the production of inflammatory cytokines during viral infection or autoimmunity (9, 10). In humans, proteolytic removal of the C-terminal 53 amino acids generates a shortened DcR3 that retains the ability to block LIGHT but not Fas Ligand induced apoptosis (11). DcR3 can also induce osteoclast formation from monocytes (12).

References

Siakavellas, S.I. and G. Bamias (2015) Inflamm. Bowel Dis. 21:2441.
Pitti, R.M. et al. (1998) Nature 396:699.
Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733.
Zhang, J. et al. (2001) J. Clin. Invest. 107:1459.
Migone, T.S. et al. (2002) Immunity 16:479.
Han, B. and J. Wu (2009) J. Immunol. 183:8157.
Ge, Z. et al. (2011) Exp. Ther. Med. 2:167.
You, R.I. et al. (2008) Blood 111:1480.
Huang, M.T. et al. (2015) J. Mol. Med. (Berlin) 93:1131.
Mueller, A.M. et al. (2009) J. Neuroimmunol. 209:57.
Wroblewski, V.J. et al. (2003) Biochem. Pharmacol. 65:657.
Yang, C.R. et al. (2004) Cell Death Differ. 11 Suppl 1:S97.

Long Name

Decoy Receptor 3

Entrez Gene IDs

8771 (Human); 107126607 (Cynomolgus Monkey)

Alternate Names

DcR3; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy