Recombinant Cynomolgus CD200R1 His-tag Protein, CF

Catalog #: 10055-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10055-CD-050

Recombinant Cynomolgus CD200R1 His-tag Protein Binding Activity

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus CD200R1 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD200 R1 is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% optimal binding response is 0.02‑0.12 μg/mL

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD200R1 protein
Ala27-Leu267, with a C-terminal 6-His tag

Accession #

XP_005548208

N-terminal Sequence
Analysis

Ala27

Predicted Molecular Mass

28 kDa

SDS-PAGE

60-67 kDa, reducing conditions

Product Datasheets

10055-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10055-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When Recombinant Cynomolgus Monkey CD200 R1 (Catalog # 10055-CD) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED₅₀ of 0.02-0.12 µg/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD200 R1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-67 kDa.

Reconstitution Calculator

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity (1-3). The cynomolgus CD200 R1 cDNA encodes a 241 amino acid (aa) extracellular domain (ECD) and 61 aa cytoplasmic tail. The ECD is composed of one Ig-like V‑type domain and one Ig-like C2-type domain (4). Within the ECD, cynomolgus CD200 R1 shares 91%, 54%, and 57% aa sequence identity with human, mouse, and rat CD200 R1, respectively. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted (5). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (6-8), while its ligand, CD200, is widely distributed (9). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (10). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (11). CD200 R1 propagates inhibitory signals despite lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (7, 8, 12, 13). CD200R1 signaling inhibits the expression of proinflammatory molecules including TNFs, IFNs, and inducible nitric oxide synthase in response to selected stimuli, which implicate that CD200/CD200R1 inhibitory signaling pathway plays a prominent role in limiting inflammation in a wide range of inflammatory diseases (14). Furthermore, the CD200/CD200R inhibitory signaling constitutes one of the most suitable endogenous immunoregulatory molecule candidate to restore the immune suppressive status of the CNS altered in chronic neuroinflammatory situations (15).

References

Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
Wright, G.J. et al. (2003) J. Immunol. 171:3034.
Vieites, J.M. et al. (2003) Gene. Jun. 5; 311:99.
Shiratori, I. et al. (2005) J. Immunol. 175:4441.
Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
Fallarino, F. et al. (2004) J. Immunol. 173:3748.
Wright, G.J. et al. (2001) Immunology 102:173.
Hoek, R.M. et al. (2000) Science 290:1768.
Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
Zhang, S. et al. (2004) J. Immunol. 173:6786.
Vaine, C.A. et al. (2014) Adv Immunol.121: 191.
Hernangómez, M. et al. (2014) Curr Pharm Des. 20:4707.

Long Name

CD200 Receptor 1

Entrez Gene IDs

131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

Alternate Names

CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor