Recombinant B. thetaiotaomicron Heparinase I Protein, CF
Recombinant B. thetaiotaomicron Heparinase I Protein, CF Summary
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5830-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Citrate. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
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Background: Heparinase I
Heparin and heparan sulfate are sulfated glycosaminoglycans that share basic carbohydrate backbone structure with alternating uronic acid and N-acetylglucosamine residues (1, 2). Heparin is found in mast cells and has strong anticoagulation properties. Heparan sulfate is found on cell membrane and extracellular matrix and is involved in various biological events from cell growth, adhesion and migration to lipid metabolism. Heparin has a much higher degree of sulfation than heparan sulfate, which can be considered as a polysaccharide with regions similar to heparin interspaced with much less sulfated regions. Both heparin and heparan sulfate can be digested by heparinases, a group of bacterial lyases that are widely used as tools for processing and analyze these polysaccharides. Heparinase I from Bacteroides thetaiotaomicron (3) is a newly discovered heparinase with no activity against chondroitin sulfate and keratan sulfate (4). The enzyme readily releases tri‑sulfated and di-sulfated disaccharides from heparin and heparan sulfate.
- MacArthur, J. M. et al. (2007) J. Clin. Invest. 117:153.
- Esko, J. D. and Selleck, S. B. (2002) Annu. Rev. Biochem. 71:435.
- Xu, J. et al. (2003) Science 299:2074.
- Luo, Y. et al. (2007) Arch. Biochem. Biophys. 460:17.
Citation for Recombinant B. thetaiotaomicron Heparinase I Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.
Authors: Wu ZL, Prather B, Ethen CM
Glycobiology, 2010-12-17;21(5):625-33.
Species: Bovine, Porcine
Sample Types: Heparin, Protein
Applications: Enzyme Assay
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