Rat GABA_B R2 N-Terminus Antibody

Catalog #: AF1188
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1188
AF1188-SP

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All GABA-B R2 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat GABA_B R2 N-Terminus Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat GABA_B R2 N-Terminus in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat GABA_B R2
Trp41-Ser482 (Phe337Tyr)
Accession # O88871

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat GABA_B R2 N-Terminus

Simple Western

20 µg/mL

Rat brain tissue

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GABA_BR2 in Rat Spinal Cord. GABA_BR2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABA_BR2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Rat GABA_B R2 by Simple Western^TM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GABA_B R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Goat Anti-Rat GABA_B R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABA-B R2

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment. When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Entrez Gene IDs

9568 (Human); 83633 (Rat)

Alternate Names

G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Rat GABA_B R2 N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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The CaMKII‐dependent phosphorylation of GABAB receptors in the nucleus accumbens was involved in cocaine‐induced behavioral sensitization in rats
Authors: Ming F. Lu, Qiang Fu, Tian Y. Qiu, Jian H. Yang, Qing H. Peng, Zhen Z. Hu
CNS Neuroscience & Therapeutics
Traumatic Brain Injury Broadly Affects GABAergic Signaling in Dentate Gyrus Granule Cells
Authors: Alejandro Parga Becerra, Aric F. Logsdon, William A. Banks, Christopher B. Ransom
eNeuro
Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
Authors: Schwenk J, Perez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca M, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B
Nat Neurosci, 2015-12-21;19(2):233-42.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot