Wash Buffer Concentrate, 25X Summary
INTENDED USE & DESCRIPTION
Wash Buffer Concentrate (Part # 895126) contains 5 vials (100 mL/vial) of a 25X concentrated solution of buffered surfactant with preservative. The concentrate can be diluted to a working concentration and used for the complete removal of any unbound sample or conjugate in the wells of an assay.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have gone into solution. May turn yellow over time.
REAGENT PREPARATION
Dilute 20 mL of Wash Buffer Concentrate to a final volume of 500 mL in deionized water. Diluted Wash Buffer may be used for up to 30 days when stored at 2-8 °C.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- Caution: Wash Buffer Concentrate contains ProClin® which may cause an allergic reaction. Avoid breathing mist. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Specifications
Product Datasheets
Citations for Wash Buffer Concentrate, 25X
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein
Authors: Chen, SJ;Lu, SY;Tseng, CC;Huang, KH;Chen, TL;Fu, LM;
Biosensors 2024-05-30
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Chronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent Neurodegeneration
Authors: Munuera, I;Aragon-Navas, A;Villacampa, P;Gonzalez-Cela, MA;Subías, M;Pablo, LE;Garcia-Feijoo, J;Herrero-Vanrell, R;Garcia-Martin, E;Bravo-Osuna, I;Rodrigo, MJ;
International journal of molecular sciences 2023-12-19
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Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
Cells 2023-08-19
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Increased neutralization potency and breadth elicited by a SARS-CoV-2 mRNA vaccine forming virus-like particles
Authors: Zhang, P;Falcone, S;Tsybovsky, Y;Singh, M;Gopan, V;Miao, H;Seo, Y;Rogers, D;Renzi, I;Lai, YT;Narayanan, E;Stewart-Jones, G;Himansu, S;Carfi, A;Fauci, AS;Lusso, P;
Proceedings of the National Academy of Sciences of the United States of America 2023-07-18
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Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):. 2021-11-03
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Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
Authors: L Kang, S Smith, C Wang
Journal of colloid and interface science, 2020-12-24;588(0):101-109. 2020-12-24
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Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
Int J Nanomedicine, 2019-08-01;14(0):6117-6131. 2019-08-01
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Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
Sci Rep, 2018-07-09;8(1):10328. 2018-07-09
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Clinical validation of a blood-based classifier for diagnostic evaluation of asymptomatic individuals with pulmonary nodules
Authors: CE Birse, JL Tomic, HI Pass, WN Rom, RJ Lagier
Clin Proteomics, 2017-07-05;14(0):25. 2017-07-05
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Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
Nat Commun, 2014-04-30;5(0):3779. 2014-04-30
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Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
Authors: Sakamoto N, Rosenberg AS
J. Immunol., 2011-03-14;186(8):5004-11. 2011-03-14
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Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010-02-16;5(2):e9246. 2010-02-16
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First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2009-12-10;33(3):664-9. 2009-12-10
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Embryonic stem cells cultured in serum-free medium acquire bovine apolipoprotein B-100 from feeder cell layers and serum replacement medium.
Authors: Hisamatsu-Sakamoto M, Sakamoto N, Rosenberg AS
Stem Cells, 2007-10-18;26(1):72-8. 2007-10-18
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Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
Chest, 2007-05-15;132(1):58-63. 2007-05-15
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Stable and used for long term
Very easy to use it.
Using wash buffer completely removes any background
