Proteome Profiler Human XL Cytokine Array Kit
Proteome Profiler Human XL Cytokine Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.
Key Benefits
- Detects 105 human cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Array
The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
Adiponectin/Acrp30 | IFN-gamma | CCL2/MCP-1 |
Angiogenin | IGFBP-2 | CCL7/MCP-3 |
Angiopoietin-1 | IGFBP-3 | M-CSF |
Angiopoietin-2 | IL-1 alpha/IL-1F1 | MIF |
Apolipoprotein A1 | IL-1 beta/IL-1F2 | CXCL9/MIG |
BAFF/BLyS/TNFSF13B | IL-1ra/IL-1F3 | CCL3/CCL4 MIP-1 alpha/beta |
BDNF | IL-2 | CCL20/MIP-3 alpha |
CD14 | IL-3 | CCL19/MIP-3 beta |
CD30 | IL-4 | MMP-9 |
CD31/PECAM-1 | IL-5 | Myeloperoxidase |
CD40 Ligand/TNFSF5 | IL-6 | Osteopontin (OPN) |
Chitinase 3-like | IL-8 | PDGF-AA |
Complement Component C5/C5a | IL-10 | PDGF-AB/BB |
Complement Factor D | IL-11 | Pentraxin 3/TSF-14 |
C-Reactive Protein/CRP | IL-12 p70 | CXCL4/PF4 |
Cripto-1 | IL-13 | RAGE |
Cystatin C | IL-15 | CCL5/RANTES |
Dkk-1 | IL-16 | RBP4 |
DPPIV/CD26 | IL-17A | Relaxin-2 |
EGF | IL-18 BPa | Resistin |
CXCL5/ENA-78 | IL-19 | CXCL12/SDF-1 alpha |
Endoglin/CD105 | IL-22 | Serpin E1/PAI-1 |
EMMPRIN | IL-23 | SHBG |
Fas Ligand | IL-24 | ST2/IL1 R4 |
FGF basic | IL-27 | CCL17/TARC |
KGF/FGF-7 | IL-31 | TFF3 |
FGF-19 | IL-32 alpha/beta/gamma | TfR |
Flt-3 Ligand | IL-33 | TGF-alpha |
G-CSF | IL-34 | Thrombospondin-1 |
GDF-15 | CXCL10/IP-10 | TIM-1 |
GM-CSF | CXCL11/I-TAC | TNF-alpha |
CXCL1/GRO alpha | Kallikrein 3/PSA | uPAR |
Growth Hormone (GH) | Leptin | VCAM-1 |
HGF | LIF | VEGF |
ICAM-1/CD54 | Lipocalin-2/NGAL | Vitamin D BP |
Specifications
Product Datasheets
Scientific Data
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
Citations for Proteome Profiler Human XL Cytokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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LUBAC enables tumor-promoting LT? receptor signaling by activating canonical NF-?B
Authors: Chen, YG;Rieser, E;Bhamra, A;Surinova, S;Kreuzaler, P;Ho, MH;Tsai, WC;Peltzer, N;de Miguel, D;Walczak, H;
Cell death and differentiation 2024-08-30
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P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance
Authors: Jiang, X;Baig, AH;Palazzo, G;Del Pizzo, R;Bortecen, T;Groessl, S;Zaal, EA;Amaya Ramirez, CC;Kowar, A;Aviles-Huerta, D;Berkers, CR;Palm, W;Tschaharganeh, D;Krijgsveld, J;Loayza-Puch, F;
Nature communications 2024-08-28
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Role of Mitofusin 1 in mediating reactive oxygen species in alveolar macrophages during Streptococcuspneumoniae
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Redox biology 2024-08-27
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Regulatory Effects of Senescent Mesenchymal Stem Cells: Endotheliocyte Reaction
Authors: Ratushnyy, A;Ezdakova, M;Matveeva, D;Tyrina, E;Buravkova, L;
Cells 2024-08-13
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Comparative Analysis of Viral Load and Cytokines during SARS-CoV-2 Infection between Pregnant and Non-Pregnant Women
Authors: Liu, D;Li, H;Li, X;Rodriguez, GD;Pietz, H;Fiel, RH;Konadu, E;Singh, V;Loo, F;Rodgers, WH;
International journal of molecular sciences 2024-07-15
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Functional Characterisation of Surfactant Protein A as a Novel Prophylactic Means against Oncogenic HPV Infections
Authors: Carse, S;Reid, T;Madsen, J;Clark, H;Kirjakulov, A;Bergant Marui?, M;Schäfer, G;
International journal of molecular sciences 2024-07-14
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The TOX-RAGE axis mediates inflammatory activation and lung injury in severe pulmonary infectious diseases
Authors: Kim, H;Park, HH;Kim, HN;Seo, D;Hong, KS;Jang, JG;Seo, EU;Kim, IY;Jeon, SY;Son, B;Cho, SW;Kim, W;Ahn, JH;Lee, W;
Proceedings of the National Academy of Sciences of the United States of America 2024-06-25
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Partial recovery of peripheral blood monocyte subsets in head and neck squamous cell carcinoma patients upon radio(chemo)therapy is associated with decreased plasma CXCL11
Authors: Idel, C;Fleckner, J;Plötze-Martin, K;Werner, L;Rades, D;Theodoraki, MN;Hofmann, L;Huber, D;Leichtle, A;Hoffmann, TK;Bruchhage, KL;Pries, R;
BMC cancer 2024-04-12
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IL-8 Instructs Macrophage Identity in Lateral Ventricle Contacting Glioblastoma
Authors: Medina, S;Brockman, AA;Cross, CE;Hayes, MJ;Mobley, BC;Mistry, AM;Chotai, S;Weaver, KD;Thompson, RC;Chambless, LB;Ihrie, RA;Irish, JM;
bioRxiv : the preprint server for biology 2024-03-30
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Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses
Authors: Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS;
Biomedicines 2024-03-25
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Spatiotemporal Clusters of ERK Activity Coordinate Cytokine-induced Inflammatory Responses in Human Airway Epithelial Cells
Authors: DeCuzzi, NL;Oberbauer, D;Chmiel, KJ;Pargett, M;Ferguson, JM;Murphy, D;Zeki, AA;Albeck, JG;
bioRxiv : the preprint server for biology 2024-02-04
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Characteristics of the synovial microenvironment and synovial mesenchymal stem cells with hip osteoarthritis of different bone morphologies
Authors: Yang, Y;Koga, H;Nakagawa, Y;Nakamura, T;Katagiri, H;Takada, R;Katakura, M;Tsuji, K;Sekiya, I;Miyatake, K;
Arthritis research & therapy 2024-01-10
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Innate Immune Activation and Mitochondrial ROS Invoke Persistent Cardiac Conduction System Dysfunction after COVID-19
Authors: Ashok, D;Liu, T;Criscione, J;Prakash, M;Kim, B;Chow, J;Craney, M;Papanicolaou, KN;Sidor, A;Brian Foster, D;Pekosz, A;Villano, J;Kim, DH;O'Rourke, B;
bioRxiv : the preprint server for biology 2024-01-08
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Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets
Authors: Pries, R;Kosyna, FK;Depping, R;Plötze-Martin, K;Lange, C;Meyhöfer, S;Meyhöfer, SM;Marquardt, JU;Bruchhage, KL;Steffen, A;
Scientific reports 2024-01-03
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Overexpression of Growth Differentiation Factor 15 in Glioblastoma Stem Cells Promotes Their Radioresistance
Authors: Bentaberry-Rosa, A;Nicaise, Y;Delmas, C;Gouazé-Andersson, V;Cohen-Jonathan-Moyal, E;Seva, C;
Cancers 2023-12-20
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Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network
Authors: Sharma, D;Sharma, A;Hu, L;Chen, TA;Voon, S;Bayless, KJ;Goldman, J;Walsh, AJ;Zhao, F;
Bioactive materials 2023-12-01
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IFI16 Induced by Direct Interaction between Esophageal Squamous Cell Carcinomas and Macrophages Promotes Tumor Progression via Secretion of IL-1?
Authors: Azumi, Y;Koma, YI;Tsukamoto, S;Kitamura, Y;Ishihara, N;Yamanaka, K;Nakanishi, T;Miyako, S;Urakami, S;Tanigawa, K;Kodama, T;Nishio, M;Shigeoka, M;Kakeji, Y;Yokozaki, H;
Cells 2023-11-10
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Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells
Authors: Chrisochoidou, Y;Roy, R;Farahmand, P;Gonzalez, G;Doig, J;Krasny, L;Rimmer, EF;Willis, AE;MacFarlane, M;Huang, PH;Carragher, NO;Munro, AF;Murphy, DJ;Veselkov, K;Seckl, MJ;Moffatt, MF;Cookson, WOC;Pardo, OE;
Cell death & disease 2023-11-08
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Analysis of proteins released from osteoarthritic cartilage by compressive loading
Authors: Tsuno, H;Tanaka, N;Naito, M;Ohashi, S;Iwasawa, M;Kadoguchi, T;Mitomi, H;Matsui, T;Furukawa, H;Fukui, N;
Scientific reports 2023-10-25
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Proteomic Insights into Metastatic Breast Cancer Response to Brain Cell-Secreted Factors
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bioRxiv : the preprint server for biology 2023-10-23
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In need of age-appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomes
Authors: Ozcebe, SG;Zorlutuna, P;
Aging cell 2023-10-06
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Differential Effects of Pancreatic Cancer-Derived Extracellular Vesicles Driving a Suppressive Environment
Authors: Purushothaman, A;Oliva-Ramírez, J;Treekitkarnmongkol, W;Sankaran, D;Hurd, MW;Putluri, N;Maitra, A;Haymaker, C;Sen, S;
International journal of molecular sciences 2023-09-27
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C9orf72-ALS human iPSC microglia are pro-inflammatory and toxic to co-cultured motor neurons via MMP9
Authors: Vahsen, BF;Nalluru, S;Morgan, GR;Farrimond, L;Carroll, E;Xu, Y;Cramb, KML;Amein, B;Scaber, J;Katsikoudi, A;Candalija, A;Carcolé, M;Dafinca, R;Isaacs, AM;Wade-Martins, R;Gray, E;Turner, MR;Cowley, SA;Talbot, K;
Nature communications 2023-09-22
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Age-related STAT3 signaling regulates severity of respiratory syncytial viral infection in human bronchial epithelial cells
Authors: Zhao, C;Wang, W;Bai, Y;Amonkar, G;Mou, H;Olejnik, J;Hume, AJ;Mühlberger, E;Fang, Y;Que, J;Fearns, R;Ai, X;Lerou, PH;
bioRxiv : the preprint server for biology 2023-09-21
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Clonal MDS/AML cells with enhanced TWIST1 expression reprogram the differentiation of bone marrow MSCs
Authors: Li, H;Wang, Y;Yang, F;Feng, S;Chang, K;Yu, X;Guan, F;Li, X;
Redox biology 2023-09-21
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Free Bilirubin Induces Neuro-Inflammation in an Induced Pluripotent Stem Cell-Derived Cortical Organoid Model of Crigler-Najjar Syndrome
Authors: Pranty, AI;Wruck, W;Adjaye, J;
Cells 2023-09-14
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Telomere dysfunction promotes cholangiocyte senescence and biliary fibrosis in Primary Sclerosing Cholangitis
Authors: Jalan-Sakrikar, N;Anwar, A;Yaqoob, U;Gan, C;Lagnado, AB;Wixom, AQ;Jurk, D;Huebert, RC;
JCI insight 2023-09-14
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Human lncRNA SUGCT-AS1 Regulates the Proinflammatory Response of Macrophage
Authors: Lim, YH;Yoon, G;Ryu, Y;Jeong, D;Song, J;Kim, YS;Ahn, Y;Kook, H;Kim, YK;
International journal of molecular sciences 2023-08-28
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Herpes Simplex Virus Infection Alters the Immunological Properties of Adipose-Tissue-Derived Mesenchymal-Stem Cells
Authors: Kun-Varga, A;Gubán, B;Miklós, V;Parvaneh, S;Guba, M;Sz?cs, D;Monostori, T;Varga, J;Varga, Á;Rázga, Z;Bata-Csörg?, Z;Kemény, L;Megyeri, K;Veréb, Z;
International journal of molecular sciences 2023-07-26
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Atezolizumab Induces Necroptosis and Contributes to Hepatotoxicity of Human Hepatocytes
Authors: Endo, Y;Winarski, KL;Sajib, MS;Ju, A;Wu, WJ;
International journal of molecular sciences 2023-07-20
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IL1R1+ cancer-associated fibroblasts drive tumor development and immunosuppression in colorectal cancer
Authors: Koncina, E;Nurmik, M;Pozdeev, VI;Gilson, C;Tsenkova, M;Begaj, R;Stang, S;Gaigneaux, A;Weindorfer, C;Rodriguez, F;Schmoetten, M;Klein, E;Karta, J;Atanasova, VS;Grzyb, K;Ullmann, P;Halder, R;Hengstschläger, M;Graas, J;Augendre, V;Karapetyan, YE;Kerger, L;Zuegel, N;Skupin, A;Haan, S;Meiser, J;Dolznig, H;Letellier, E;
Nature communications 2023-07-17
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Endocrine therapy synergizes with SMAC mimetics to potentiate antigen presentation and tumor regression in hormone receptor-positive breast cancer
Authors: Hermida-Prado, F;Xie, Y;Sherman, S;Nagy, Z;Russo, D;Akhshi, T;Chu, Z;Feit, A;Campisi, M;Chen, M;Nardone, A;Guarducci, C;Lim, K;Font-Tello, A;Lee, I;García-Pedrero, J;Canadas, I;Agudo, J;Huang, Y;Sella, T;Jin, Q;Tayob, N;Mittendorf, EA;Tolaney, SM;Qiu, X;Long, H;Symmans, WF;Lin, JR;Santagata, S;Bedrosian, I;Yardley, DA;Mayer, IA;Richardson, ET;Oliveira, G;Wu, CJ;Schuster, EF;Dowsett, M;Welm, AL;Barbie, D;Metzger, O;Jeselsohn, R;
Cancer research 2023-07-14
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Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia
Authors: Park, CS;Yoshihara, H;Gao, Q;Qu, C;Iacobucci, I;Ghate, PS;Connelly, JP;Pruett-Miller, SM;Wagner, B;Robinson, CG;Mishra, A;Peng, J;Yang, L;Rankovic, Z;Finkelstein, D;Luger, S;Litzow, M;Paietta, EM;Hebbar, N;Velasquez, MP;Mullighan, CG;
Cell reports 2023-07-14
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HMGB1, a potential regulator of tumor microenvironment in KSHV-infected endothelial cells
Authors: Lee, MJ;Park, J;Choi, S;Yoo, SM;Park, C;Kim, HS;Lee, MS;
Frontiers in microbiology 2023-07-13
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Simultaneous isolation of hormone receptor positive breast cancer organoids and fibroblasts reveals stroma-mediated resistance mechanisms
Authors: Hogstrom, JM;Cruz, KA;Selfors, LM;Ward, MN;Mehta, TS;Kanarek, N;Philips, J;Dialani, V;Wulf, G;Collins, LC;Patel, JM;Muranen, T;
The Journal of biological chemistry 2023-07-07
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Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome
Authors: Cozzolino, K;Sanford, L;Hunter, S;Molison, K;Erickson, B;Jones, T;Ajit, D;Galbraith, MD;Espinosa, JM;Bentley, DL;Allen, MA;Dowell, RD;Taatjes, DJ;
bioRxiv : the preprint server for biology 2023-07-05
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Gene Expression Changes in Cytokine and Chemokine Receptors in Association with Melanoma Liver Metastasis
Authors: Koroknai, V;Sz�sz, I;Bal�zs, M;
International journal of molecular sciences 2023-05-17
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TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseases
Authors: Kim, J;Lim, J;Yoo, ID;Park, S;Moon, JS;
Redox biology 2023-05-06
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Self-assembled peptide-substance P hydrogels alleviate inflammation and ameliorate the cartilage regeneration in knee osteoarthritis
Authors: Kim, SJ;Kim, JE;Choe, G;Song, DH;Kim, SJ;Kim, TH;Yoo, J;Kim, SH;Jung, Y;
Biomaterials research 2023-05-04
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Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells
Authors: AL Pourzia, ML Olson, SR Bailey, AC Boroughs, A Aryal, J Ryan, MV Maus, A Letai
Cell Death & Disease, 2023-04-13;14(4):267. 2023-04-13
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miRNome and Proteome Profiling of Human Keratinocytes and Adipose Derived Stem Cells Proposed miRNA-Mediated Regulations of Epidermal Growth Factor and Interleukin 1-Alpha
Authors: H Shahin, S Abdallah, J Das, W He, I El-Serafi, I Steinvall, F Sjöberg, M Elmasry, AT El-Serafi
International Journal of Molecular Sciences, 2023-03-04;24(5):. 2023-03-04
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Extracellular Vesicles Derived from Three-Dimensional-Cultured Human Umbilical Cord Blood Mesenchymal Stem Cells Prevent Inflammation and Dedifferentiation in Pancreatic Islets
Authors: E Lee, S Ha, G Kim, JH Kim, SM Jin
Stem Cells International, 2023-02-20;2023(0):5475212. 2023-02-20
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gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells
Authors: AJ Suh, DI Suzuki, SG Gychka, TI Brelidze, YJ Suzuki
International Journal of Molecular Sciences, 2023-02-10;24(4):. 2023-02-10
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Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury
Authors: ME Mohr, S Li, AM Trouten, RA Stairley, PL Roddy, C Liu, M Zhang, HM Sucov, G Tao
bioRxiv : the preprint server for biology, 2023-02-08;0(0):. 2023-02-08
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Tumor-secreted exosomal miR-141 activates tumor-stroma interactions and controls premetastatic niche formation in ovarian cancer metastasis
Authors: Y Mo, LL Leung, CSL Mak, X Wang, WS Chan, LMN Hui, HWM Tang, MKY Siu, R Sharma, D Xu, SKW Tsui, HYS Ngan, MMH Yung, KKL Chan, DW Chan
Molecular Cancer, 2023-01-09;22(1):4. 2023-01-09
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gp120 envelope glycoproteins of HIV-1 Group M Subtype A and Subtype B differentially affect gene expression in human vascular endothelial cells
Authors: AJ Suh, DI Suzuki, SG Gychka, TI Brelidze, YJ Suzuki
bioRxiv : the preprint server for biology, 2023-01-03;0(0):. 2023-01-03
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Pericytes take up and degrade alpha-synuclein but succumb to apoptosis under cellular stress
Authors: TJ Stevenson, RH Johnson, J Savistchen, J Rustenhove, Z Woolf, LCD Smyth, HC Murray, RLM Faull, J Correia, P Schweder, P Heppner, C Turner, R Melki, BV Dieriks, MA Curtis, M Dragunow
Scientific Reports, 2022-10-15;12(1):17314. 2022-10-15
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Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
Nature Communications, 2022-10-14;13(1):6078. 2022-10-14
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The Differential Paracrine Role of the Endothelium in Prostate Cancer Cells
Authors: V Torres-Est, M Mastri, S Rosario, P Fuenzalida, CE Echeverría, E Flores, A Watts, J Cerda-Infa, VP Montecinos, PC Sotomayor, J Amigo, C Escudero, F Nualart, JML Ebos, DJ Smiraglia, AS Godoy
Cancers, 2022-09-29;14(19):. 2022-09-29
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The Nicotinic Receptor Polymorphism rs16969968 Is Associated with Airway Remodeling and Inflammatory Dysregulation in COPD Patients
Authors: L Saber Cher, Z Diabasana, JM Perotin, J Ancel, LMG Petit, MA Devilliers, A Bonnomet, N Lalun, G Delepine, U Maskos, P Gosset, M Polette, A Muggeo, T Guillard, G Deslée, V Dormoy
Cells, 2022-09-20;11(19):. 2022-09-20
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ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model
Authors: YY Wang, AC Hung, YC Wu, S Lo, HD Chen, YK Chen, YC Hsieh, SC Hu, MF Hou, SF Yuan
Scientific Reports, 2022-09-14;12(1):15437. 2022-09-14
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Melanoma Mediated Disruption of Brain Endothelial Barrier Integrity Is Not Prevented by the Inhibition of Matrix Metalloproteinases and Proteases
Authors: A Anchan, G Finlay, CE Angel, JJW Hucklesby, SE Graham
Biosensors, 2022-08-19;12(8):. 2022-08-19
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During early stages of cancer, neutrophils initiate anti-tumor immune responses in tumor-draining lymph nodes
Authors: E Pylaeva, G Korschunow, I Spyra, S Bordbari, E Siakaeva, I Ozel, M Domnich, A Squire, A Hasenberg, K Thangavelu, T Hussain, M Goetz, KS Lang, M Gunzer, W Hansen, J Buer, A Bankfalvi, S Lang, J Jablonska
Cell Reports, 2022-08-16;40(7):111171. 2022-08-16
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Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma
Authors: M Giannaki, DE Ruf, E Pfeifer, K Everaerts, DH Heiland, O Schnell, CR Rose, E Roussa
International Journal of Molecular Sciences, 2022-08-11;23(16):. 2022-08-11
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Plasmid-mediated gene transfer of Cas9 induces vector-related but not SpCas9-related immune responses in human retinal pigment epithelial cells
Authors: JK Pfromm, M Bonillo, D Dauletbeko, K Bucher, MD Fischer
Scientific Reports, 2022-08-01;12(1):13202. 2022-08-01
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Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
Scientific Reports, 2022-07-23;12(1):12606. 2022-07-23
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Targeting HIC1/TGF-beta axis-shaped prostate cancer microenvironment restrains its progression
Authors: T Wu, W Wang, G Shi, M Hao, Y Wang, M Yao, Y Huang, L Du, X Zhang, D Ye, X Bian, J Wang
Cell Death & Disease, 2022-07-19;13(7):624. 2022-07-19
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Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance
Authors: R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta
Oncogene, 2022-07-19;40(3):111097. 2022-07-19
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Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells
Authors: N Igarashi, K Miyata, TM Loo, M Chiba, A Hanyu, M Nishio, H Kawasaki, H Zheng, S Toyokuni, S Kon, K Moriyama, Y Fujita, A Takahashi
Oncogene, 2022-07-18;13(1):4157. 2022-07-18
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Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
Authors: H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd
Mucosal Immunology, 2022-07-15;0(0):. 2022-07-15
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The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cells
Authors: D Ávila-Gonz, W Portillo, CP Barragán-Á, G Hernandez-, E Flores-Gar, A Molina-Her, NE Díaz-Martí, NF Díaz
Elife, 2022-07-11;11(0):. 2022-07-11
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Interleukin-8 produced from cancer-associated fibroblasts suppresses proliferation of the OCUCh-LM1 cancer cell line
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The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues
Authors: Y Wada, T Ikemoto, Y Morine, S Imura, Y Saito, S Yamada, M Shimada
Sci Rep, 2019-09-13;9(1):13204. 2019-09-13
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The Secretomes of Painful Versus Nonpainful Human Schwannomatosis Tumor Cells Differentially Influence Sensory Neuron Gene Expression and Sensitivity
Authors: KL Ostrow, KJ Donaldson, MJ Caterina, A Belzberg, A Hoke
Sci Rep, 2019-09-11;9(1):13098. 2019-09-11
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Radiotherapy-Induced Changes in the Systemic Immune and Inflammation Parameters of Head and Neck Cancer Patients
Authors: K Balázs, E Kis, C Badie, EN Bogdándi, S Candéias, LC Garcia, I Dominczyk, B Frey, U Gaipl, Z Jurányi, ZS Kocsis, EA Rutten, G Sáfrány, P Widlak, K Lumniczky
Cancers (Basel), 2019-09-06;11(9):. 2019-09-06
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Hypoxia Rapidly Induces the Expression of Cardiomyogenic Factors in Human Adipose-Derived Adherent Stromal Cells
Authors: JW Choi, H Moon, SE Jung, S Lim, S Lee, IK Kim, HB Lee, J Lee, BW Song, SW Kim, KC Hwang
J Clin Med, 2019-08-15;8(8):. 2019-08-15
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Obesity in acute alcoholic hepatitis increases morbidity and mortality
Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
EBioMedicine, 2019-07-03;45(0):511-518. 2019-07-03
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Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastoma
Authors: D Henrik Hei, VM Ravi, SP Behringer, JH Frenking, J Wurm, K Joseph, NWC Garrelfs, J Strähle, S Heynckes, J Grauvogel, P Franco, I Mader, M Schneider, AL Potthoff, D Delev, UG Hofmann, C Fung, J Beck, R Sankowski, M Prinz, O Schnell
Nat Commun, 2019-06-11;10(1):2541. 2019-06-11
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Paradoxical effects of JZL184, an inhibitor of monoacylglycerol lipase, on bone remodelling in healthy and cancer-bearing mice
Authors: S Marino, D de Ridder, RT Bishop, N Renema, M Ponzetti, A Sophocleou, M Capulli, A Aljeffery, G Carrasco, MD Gens, A Khogeer, SH Ralston, J Gertsch, F Lamoureux, D Heymann, N Rucci, AI Idris
EBioMedicine, 2019-05-29;44(0):452-466. 2019-05-29
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p16INK4A-expressing mesenchymal stromal cells restore the senescence-clearance-regeneration sequence that is impaired in chronic muscle inflammation
Authors: TS Chikenji, Y Saito, N Konari, M Nakano, Y Mizue, M Otani, M Fujimiya
EBioMedicine, 2019-05-23;0(0):. 2019-05-23
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Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature
Authors: LS Spitzhorn, M Megges, W Wruck, MS Rahman, J Otte, Ö Degistiric, R Meisel, RV Sorg, ROC Oreffo, J Adjaye
Stem Cell Res Ther, 2019-03-18;10(1):100. 2019-03-18
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Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis
Authors: W Liu, P Wang, Z Xie, S Wang, M Ma, J Li, M Li, S Cen, S Tang, G Zheng, G Ye, X Wu, Y Wu, H Shen
Cell Death Dis, 2019-02-25;10(3):188. 2019-02-25
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Blockage of TGF-? Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells
Authors: Y Li, H Li, Y Duan, X Cai, D You, F Zhou, C Yang, X Tuo, Z Liu
Biomed Res Int, 2019-02-18;2019(0):8231267. 2019-02-18
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The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method
Authors: D Kardos, M Simon, G Vácz, A Hinsenkamp, T Holczer, D Cseh, A Sárközi, K Szenthe, F Bánáti, S Szathmary, S Nehrer, O Kuten, M Masteling, Z Lacza, I Hornyák
Int J Mol Sci, 2019-02-08;20(3):. 2019-02-08
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Ectoine-Containing Inhalation Solution versus Saline Inhalation Solution in the Treatment of Acute Bronchitis and Acute Respiratory Infections: A Prospective, Controlled, Observational Study
Authors: BH Tran, VA Dao, A Bilstein, K Unfried, K Shah-Hosse, R Mösges
Biomed Res Int, 2019-01-31;2019(0):7945091. 2019-01-31
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A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
Cell Rep, 2018-12-26;25(13):3706-3720.e8. 2018-12-26
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Genetic and pharmacological validation of TAK1 inhibition in macrophages as a therapeutic strategy to effectively inhibit TNF secretion
Authors: SA Scarneo, A Mansourati, LS Eibschutz, J Totzke, JR Roques, D Loiselle, D Carlson, P Hughes, TAJ Haystead
Sci Rep, 2018-11-19;8(1):17058. 2018-11-19
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VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology, 2018-10-10;156(1):74-85. 2018-10-10
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USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
Authors: YC Wang, YS Wu, CY Hung, SA Wang, MJ Young, TI Hsu, JJ Hung
Nat Commun, 2018-09-28;9(1):3996. 2018-09-28
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Ca2+ Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
Authors: K Eubler, C Herrmann, A Tiefenbach, FM Köhn, JU Schwarzer, L Kunz, A Mayerhofer
Int J Mol Sci, 2018-09-19;19(9):. 2018-09-19
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Gle1 mediates stress granule-dependent survival during chemotoxic stress
Authors: L Glass, SR Wente
Adv Biol Regul, 2018-09-18;0(0):. 2018-09-18
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Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
Int J Med Sci, 2018-09-07;15(12):1365-1372. 2018-09-07
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Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
Cell Rep, 2018-08-28;24(9):2300-2311. 2018-08-28
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A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
J Oral Sci, 2018-08-25;0(0):. 2018-08-25
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Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
Nat Commun, 2018-08-15;9(1):3267. 2018-08-15
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Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
Cancer Med, 2018-08-09;0(0):. 2018-08-09
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Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
Front Physiol, 2018-07-31;9(0):1025. 2018-07-31
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Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
J. Cell. Mol. Med., 2018-07-05;0(0):. 2018-07-05
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IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
Nature, 2018-06-27;0(0):. 2018-06-27
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Unique and shared inflammatory profiles of human brain endothelia and pericytes
Authors: LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow
J Neuroinflammation, 2018-05-11;15(1):138. 2018-05-11
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Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
Cell Death Dis, 2018-05-01;9(5):506. 2018-05-01
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CST3 and GDF15 ameliorate renal fibrosis by inhibiting fibroblast growth and activation
Authors: YI Kim, HW Shin, YS Chun, JW Park
Biochem. Biophys. Res. Commun., 2018-04-13;0(0):. 2018-04-13
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Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro
Authors: MD Wood, J Mukherjee, RO Pieper
Sci Rep, 2018-04-11;8(1):5805. 2018-04-11
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Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
Am. J. Pathol., 2018-04-01;188(4):950-966. 2018-04-01
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Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
Sci Adv, 2018-02-21;4(2):e1701729. 2018-02-21
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Withaferin A Associated Differential Regulation of Inflammatory Cytokines
Authors: S Dubey, H Yoon, MS Cohen, P Nagarkatti, M Nagarkatti, D Karan
Front Immunol, 2018-02-09;9(0):195. 2018-02-09
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Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
Cell Death Dis, 2018-02-05;9(2):154. 2018-02-05
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ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
Authors: L Walenta, D Fleck, T Fröhlich, H von Eysmon, GJ Arnold, J Spehr, JU Schwarzer, FM Köhn, M Spehr, A Mayerhofer
Sci Rep, 2018-01-23;8(1):1431. 2018-01-23
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Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
Authors: JM Cha, EK Shin, JH Sung, GJ Moon, EH Kim, YH Cho, HD Park, H Bae, J Kim, OY Bang
Sci Rep, 2018-01-19;8(1):1171. 2018-01-19
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Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts
Authors: T Kifune, H Ito, M Ishiyama, S Iwasa, H Takei, T Hasegawa, M Asano, T Shirakawa
J Oral Sci, 2018-01-01;60(4):519-525. 2018-01-01
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Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype
Authors: AL Strong, DT Pei, CG Hurst, JM Gimble, ME Burow, BA Bunnell
Stem Cells Int, 2017-12-17;2017(0):9216502. 2017-12-17
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Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
Authors: BP Applewhite, S Babapoor-F, D Poon, SJ Hassan, E Wellmann, HS Ying, GL Semenza, S Montaner, A Sodhi
Sci Rep, 2017-09-06;7(1):10608. 2017-09-06
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Histone Deacetylase 1 Depletion Activates Human Cardiac Mesenchymal Stromal Cell Proangiogenic Paracrine Signaling Through a Mechanism Requiring Enhanced Basic Fibroblast Growth Factor Synthesis and Secretion
Authors: JB Moore, J Zhao, AG Fischer, MCL Keith, D Hagan, M Wysoczynsk, R Bolli
J Am Heart Assoc, 2017-07-05;6(7):. 2017-07-05
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Protective Effect of Let-7 miRNA Family in Regulating Inflammation in Diabetes-Associated Atherosclerosis
Authors: E Brennan, B Wang, A McClelland, M Mohan, M Marai, O Beuscart, S Derouiche, S Gray, R Pickering, C Tikellis, M de Gaetano, M Barry, O Belton, ST Ali-Shah, P Guiry, KA Jandeleit-, ME Cooper, C Godson, P Kantharidi
Diabetes, 2017-05-09;0(0):. 2017-05-09
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Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
J Am Heart Assoc, 2017-05-03;6(5):. 2017-05-03
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An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
Oncotarget, 2017-04-11;8(15):24869-24881. 2017-04-11
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Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
J. Immunol., 2017-04-10;0(0):. 2017-04-10
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Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis
Authors: K Jin, NB Pandey, AS Popel
Oncotarget, 2017-01-01;0(0):. 2017-01-01
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Pretreatment of IL-1? enhances proliferation and chondrogenic potential of synovium-derived mesenchymal stem cells
Authors: Etsuko Matsumura
Cytotherapy, 2016-12-12;0(0):. 2016-12-12
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Transcriptional upregulation of c-MET is associated with invasion and tumor budding in colorectal cancer
Oncotarget, 2016-11-29;0(0):. 2016-11-29
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Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
Sci Rep, 2016-07-12;6(0):29588. 2016-07-12
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Identification of Pathways in Liver Repair Potentially Targeted by Secretory Proteins from Human Mesenchymal Stem Cells
Int J Mol Sci, 2016-07-09;17(7):. 2016-07-09
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Diesel Exhaust Particles Upregulate Interleukins IL-6 and IL-8 in Nasal Fibroblasts
Authors: Jin Ah Kim
PLoS ONE, 2016-06-13;11(6):e0157058. 2016-06-13
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Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer
Nature, 2016-05-18;533(7604):493-8. 2016-05-18
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Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
PLoS Pathog, 2015-12-08;11(12):e1005319. 2015-12-08
FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
Reviews for Proteome Profiler Human XL Cytokine Array Kit
Average Rating: 4.7 (Based on 20 Reviews)
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Product was easy to use and quick. In house analysis software would improve the process. Array has not been tested if the specific antibodies differentiate between precursor and mature proteins or bound vs unbound proteins. Testing this would increase applicability. Image provided is an example of 100 ul of human serum.
Used 1 ml of pooled basal media from air-liquid interface cultures of primary epithelial cells
Protocol very easy to follow, but data analysis can be tricky. Would benefit massively from coming with its own analysis tool / ImageJ plugin.
220 uL of rat plasma for overnight incubation with arrays at 4c.
Human endothelial cells treated +/- FAK kinase inhibitors +/- doxorubicin
Chiavari M, Ciotti GMP, Navarra P, Lisi L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019;9(5):111. Published 2019 May 15. doi:10.3390/brainsci9050111
THP-1 Cells stimulated with LPS+IFN gamma for 24 hours.