Phospho-Tyrosine Agarose-conjugated Antibody

Catalog #: AGM1676 Datasheet / COA / SDS

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AGM1676

Immunoprecipitation of Human Phospho-tyrosine.

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Phospho-Tyrosine Agarose-conjugated Antibody Summary

Specificity

Detects endogenous proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using ligand- and pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Source

Monoclonal Mouse IgG1 Clone # 179003

Formulation

Phosphate-buffered saline (PBS) with preservative.

Label

Agarose(Excitation= nm, Emission= nm)

Applications

Recommended Concentration

Sample

Immunoprecipitation

10 µL/500 µg cell lysate

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human Phospho-tyrosine. A431 human epithelial carcinoma cell line was untreated (-) or treated (+) with 200 mM pervanadate (PV) for 15 minutes. Phospho-tyrosine was immunoprecipitated from 500 µg of cell lysate following incubation with 10 µg Mouse Anti-Phospho-Tyrosine Agarose-conjugated Monoclonal Antibody (Catalog # AGM1676) for 16 hours at 2 - 8 ºC. Immunoprecipitated phospho-tyrosine was detected by Western blot using 1 µg Phospho-Tyrosine Biotinylated Monoclonal Antibody (left panel, Catalog # BAM1676) or Human EGF R/ErbB1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (right panel, Catalog # BAF231). View our recommended buffer recipes for immunoprecipitation.

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Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; pY20; Tyrosine