Home / Protein Kinase C Activators / Phorbol 12,13-dibutyrate

Phorbol 12,13-dibutyrate

Catalog #: 4153
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4153/1
4153/10
Phorbol 12,13-dibutyrate | CAS No. 37558-16-0 | Protein Kinase C Activators
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Description: Protein kinase C activator
Alternative Names: PDBu

Chemical Name: (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester

Purity: ≥97%

Product Details
Citations (11)
Reviews
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells.

For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs

Technical Data

M.Wt:
504.61
Formula:
C28H40O8
Solubility:
Soluble to 50 mM in DMSO
Purity:
≥97%
Storage:
Store at -20°C
CAS No:
37558-16-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines.
    Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, Greiner D, Keller G
    Stem Cell Reports, 2015;4(4):591-604.
  2. Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells.
    Middleton et al.
    J.Biol.Chem., 1993;268:15958
  3. Presynaptic mechanism for phorphol ester-induced synaptic potentiation.
    Hori et al.
    J.Neurosci., 1999;19:7262
  4. Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness.
    Sakai et al.
    J.Smooth Muscle Res., 2010;46:259

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Citations for Phorbol 12,13-dibutyrate

The citations listed below are publications that use Tocris products. Selected citations for Phorbol 12,13-dibutyrate include:

11 Citations: Showing 1 - 10

  1. Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching.
    Authors: Gareth E Et al.
    Sci Adv  2022;8:eabm3471
  2. Do more with Less: Improving High Parameter Cytometry Through Overnight Staining.
    Authors: Oliver T Et al.
    Curr Protoc  2022;2:e589
  3. A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice.
    Authors: Sarah C Et al.
    Med (N Y)  2021;2:243-262.e8
  4. Differential Regulation of ATP- and UTP-Evoked Prostaglandin E2 and IL-6 Production from Human Airway Epithelial Cells.
    Authors: Timothy S Et al.
    J Immunol  2021;207:1275-1287
  5. Neonatal-derived IL-17 producing dermal γδ T cells are required to prevent spontaneous atopic dermatitis.
    Authors: Nidhi Et al.
    Elife  2020;9
  6. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
    Authors: Zoltan Et al.
    Cell Rep  2020;32:107968
  7. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells.
    Authors: Xavier Et al.
    Cell Death Dis  2020;11:378
  8. Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations.
    Authors: Nieuwenhuijze Et al.
    Front Immunol  2018;9:2234
  9. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines.
    Authors: Nostro Et al.
    J Cell Biol  2015;4:591
  10. Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from Ins-resistant obese rats: role of ET(A) and ET(B) receptors.
    Authors: Sánchez Et al.
    Br J Pharmacol  2014;171:5682

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