Non-Human Primate CCL19/MIP-3b Cartridge

Catalog #: SPCKB-NP-011294 Datasheet / COA / SDS
Simple Plex Non-Human Primate MIP-3b assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-NP-011294
SPCKA-NP-011294
SPCKC-NP-011294
Control Products Available
Non-Human Primate CCL19/MIP-3b Ella Assay Standard Curve
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Non-Human Primate CCL19/MIP-3b Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.35 pg/mL
Assay Range
3.9 - 6,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant MIP-3 beta. 
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of non-human primate Macrophage Inflammatory Protein 3 beta (MIP-3 beta ) in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 37 36
Mean (pg/mL) 36.4 2055 34.8 1881
Standard Deviation 1.57 115 3.51 209
CV% 4.3 5.6 10.1 11.1

Recovery

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 86 79-101
Heparin Plasma (n=5) 82 78-88
Serum (n=5) 81 76-89

Linearity

Samples containing and/or spiked with high concentrations of MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Non-Human Primate CCL19/MIP-3b Ella Assay Linearity

Scientific Data

Non-Human Primate CCL19/MIP-3b Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs:
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names:
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

FAQs

No product specific FAQs exist for this product, however you may

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