Necrostatin-1
Chemical Name: 5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone
Purity: ≥98%
Biological Activity
Necrostatin-1 is an ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC50 = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC50 = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis
SW Alvarez, VO Sviderskiy, EM Terzi, T Papagianna, AL Moreira, S Adams, DM Sabatini, K Birsoy, R Possemato
Nature, 2017;551(7682):639-643. -
Rhinovirus 3C protease suppresses apoptosis and triggers caspase-independent cell death
M Lötzerich, PS Roulin, K Boucke, R Witte, O Georgiev, UF Greber
Cell Death Dis, 2018;9(3):272. -
DEBIO 1143, an IAP inhibitor, reverses carboplatin resistance in ovarian cancer cells and triggers apoptotic or necroptotic cell death
B Thibault, L Genre, A Le Naour, C Broca, E Mery, G Vuagniaux, JP Delord, N Wiedemann, B Couderc
Sci Rep, 2018;8(1):17862. -
Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells
HJ Kim, KE Hwang, DS Park, SH Oh, HY Jun, KH Yoon, ET Jeong, HR Kim, YS Kim
J Transl Med, 2017;15(1):123. -
Cellular stress amplifies TLR3/4-induced CXCL1/2 gene transcription in mononuclear phagocytes via RIPK1.
Zhao C, Pavicic P, Datta S, Sun D, Novotny M, Hamilton T
J Immunol, 2014;193(2):879-88. -
Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury.
Degterev et al.
Nat.Chem.Biol., 2005;1:112 -
Identification of RIP1 kinase as a specific cellular target of necroSTAT.
Degterev et al.
Nat.Chem.Biol., 2008;4:313
Product Datasheets
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Citations for Necrostatin-1
The citations listed below are publications that use Tocris products. Selected citations for Necrostatin-1 include:
4 Citations: Showing 1 - 4
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A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells.
Authors: Chefetz Et al.
Cell Rep 2019;26:3061
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ONC201 kills breast cancer cells in vitro by targeting mitochondria.
Authors: Greer Et al.
Oncotarget 2018;9:18454
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Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells
Authors: Kim
Journal of Translational Medicine 2017;15(1):123
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The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells.
Authors: Sagher Et al.
Cancer Cell Int 2014;14:75
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