Mouse Vitronectin Antibody

Catalog #: MAB38751
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB38751
MAB38751-SP

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All Vitronectin Antibodies

Product Details

Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Vitronectin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Vitronectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Vitronectin is observed.

Source

Monoclonal Rat IgG_2A Clone # 347317

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Vitronectin
Asp20-Lys478
Accession # AAH12690

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Vitronectin in Mouse Brain. Vitronectin was detected in immersion fixed frozen sections of fetal mouse brain using 10 µg/mL Rat Anti-Mouse Vitronectin Monoclonal Antibody (Catalog # MAB38751) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitronectin

Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha _v beta ₃) and thrombin. The 459 amino acid (aa) mature mouse Vitronectin shows 74% aa sequence identity with human Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.

Entrez Gene IDs

7448 (Human); 22370 (Mouse); 507525 (Bovine)

Alternate Names

Complement S-protein; epibolin; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; S-protein; V75; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin; VN; VNT; VTN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Vitronectin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Suppression of CEBPdelta recovers exhaustion in anti-metastatic immune cells
Authors: C Yin, M Kato, T Tomita, Y Han, S Hiratsuka
Scientific Reports, 2023-03-08;13(1):3903.
Species: Mouse
Sample Types: Whole Tissue
Precapillary sphincters maintain perfusion in the cerebral cortex
Authors: S Grubb, C Cai, BO Hald, L Khennouf, RP Murmu, AGK Jensen, J Fordsmann, S Zambach, M Lauritzen
Nat Commun, 2020-01-20;11(1):395.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels
Authors: Z Bagi, Y Couch, Z Broskova, F Perez-Bald, T Yeo, S Davis, R Fischer, NR Sibson, BG Davis, DC Anthony
Sci Rep, 2019-11-01;9(1):15847.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in theï¿½lung
Authors: S Hiratsuka, T Tomita, T Mishima, Y Matsunaga, T Omori, S Ishibashi, S Yamaguchi, T Hosogane, H Watarai, M Omori-Miya, T Yamamoto, N Shibata, A Watanabe, H Aburatani, M Tomura, KA High, Y Maru
EMBO Mol Med, 2018-07-01;10(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
Authors: Wang X, Rodda L, Bannard O, Cyster J
J Immunol, 2014-04-16;192(10):4601-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.
Authors: Franco M, Roswall P, Cortez E, Hanahan D, Pietras K
Blood, 2011-07-21;118(10):2906-17.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Age-related retinopathy in NRF2-deficient mice.
Authors: Zhao Z, Chen Y, Wang J
PLoS ONE, 2011-04-29;6(4):e19456.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr