Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

Catalog #: MVR200B
14 Citations Datasheet / COA / SDS

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MVR200B

Mouse VEGF R2/KDR/Flk-1 ELISA Standard Curve

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All VEGFR2/KDR/Flk-1 ELISAs

Product Details

Citations (14)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse VEGFR2/Flk-1 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.049 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse sVEGF R2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse soluble VEGF R2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	24	24	24
Mean (ng/mL)	0.63	1.4	3.83	0.57	1.28	3.5
Standard Deviation	0.04	0.07	0.21	0.06	0.1	0.21
CV%	6.3	5	5.5	10.5	7.8	6

Recovery

The recovery of mouse sVEGF R2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	105	100-111
Plasma (n=4)	108	89-116
Serum (n=3)	104	82-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sVEGF R2 in each matrix were diluted with Calibrator Diluent and then assayed.

Scientific Data

N/A

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Mouse VEGF R2/KDR/Flk-1 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR2/KDR/Flk-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Long Name:

Vascular Endothelial Growth Factor Receptor 2

Entrez Gene IDs:

3791 (Human); 16542 (Mouse)

Alternate Names:

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
Mol. Cancer Ther., 2018-04-25;0(0):.
Species: Mouse
Sample Types: Plasma
Vascular Endothelial Growth Factor Sequestration Enhances In Vivo Cartilage Formation
Authors: CM Medeiros D, V Perugini, P Bernegger, M Centola, A Barbero, AL Guildford, M Santin, A Banfi, I Martin, A Marsano
Int J Mol Sci, 2017-11-21;18(11):.
Species: Human
Sample Types: Cell Culture Supernates
Hedgehog signalling pathway orchestrates angiogenesis in triple-negative breast cancers
Authors: C Di Mauro, R Rosa, V D'Amato, P Ciciola, A Servetto, R Marciano, RC Orsini, L Formisano, S De Falco, V Cicatiello, M Di Bonito, M Cantile, F Collina, A Chambery, BM Veneziani, S De Placido, R Bianco
Br. J. Cancer, 2017-04-25;0(0):.
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Serum
Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex.
Authors: Howell KR, Kutiyanawalla A, Pillai A
PLoS ONE, 2011-05-27;6(5):e20198.
Species: Mouse
Sample Types: Serum
Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
Authors: Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C
J. Neurosci., 2010-11-10;30(45):15052-66.
Species: Mouse
Sample Types: Tissue Homogenates
(Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
Authors: Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S
Am. J. Pathol., 2008-10-30;173(6):1911-8.
Species: Mouse
Sample Types: Tissue Homogenates
Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
Authors: Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S
Invest. Ophthalmol. Vis. Sci., 2008-04-01;49(4):1679-85.
Species: Mouse
Sample Types: Tissue Homogenates
An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
Authors: Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA
FASEB J., 2008-04-01;22(8):2775-83.
Species: Mouse
Sample Types: Tissue Homogenates
Vascular endothelial growth factor-mediated decrease in plasma soluble vascular endothelial growth factor receptor-2 levels as a surrogate biomarker for tumor growth.
Authors: Ebos JM, Lee CR, Bogdanovic E, Alami J, Van Slyke P, Francia G, Xu P, Mutsaers AJ, Dumont DJ, Kerbel RS
Cancer Res., 2008-01-15;68(2):521-9.
Species: Mouse
Sample Types: Plasma
Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74.
Species: Mouse
Sample Types: Plasma

Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
Am. J. Physiol. Heart Circ. Physiol., 2007-03-16;293(1):H474-81.
Species: Rat
Sample Types: Cell Culture Supernates

Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts.
Authors: Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T
Am. J. Physiol. Heart Circ. Physiol., 2006-04-14;291(3):H1290-8.
Species: Rat
Sample Types: Tissue Homogenates

Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor.
Authors: Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S
Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):1078-84.
Species: Mouse
Sample Types: Tissue Homogenates

Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.
Authors: Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA
J. Clin. Invest., 2003-03-01;111(5):649-58.
Species: Rat
Sample Types: Plasma