Mouse TSLPR Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5461F
Detection of TSLP R in Mouse Splenocytes by Flow Cytometry.
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Citations (1)
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Mouse TSLPR Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TSLP R in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TSLP R
Ala20-Leu233
Accession # BAA92159
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TSLP R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TSLP R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TSLP R Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5461F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The TSLP R cDNA encodes a 359 amino acid (aa) residue type 1 membrane protein with a 24 aa residue signal peptide, a 206 aa residue extracellular domain that showed 24% sequence identity with the mouse common gamma receptor, a 23 aa residue transmembrane domain, and a 106 aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with JAKs. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3, 4).

References
  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Long Name
Thymic Stromal Lymphopoietin Receptor
Entrez Gene IDs
64109 (Human); 57914 (Mouse); 171499 (Rat)
Alternate Names
CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR

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Citation for Mouse TSLPR Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Thymic stromal lymphopoietin limits primary and recall CD8+ T-cell anti-viral responses
    Authors: R Ebina-Shib, EE West, R Spolski, P Li, J Oh, M Kazemian, D Gromer, P Swanson, N Du, DB McGavern, WJ Leonard
    Elife, 2021-01-13;10(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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