Mouse TCR gamma /δ Biotinylated Antibody

Catalog #: FAB7297B Datasheet / COA / SDS

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FAB7297B-100

Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry.

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Mouse TCR gamma /δ Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TCR gamma /δ in flow cytometry.

Source

Monoclonal Hamster IgG₂ kappa Clone # GL-3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse intraepithelial lymphocytes

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma/d Biotinylated Monoclonal Antibody (Catalog # FAB7297B) or (B) Hamster IgG Biotinylated Control (Catalog # BAM011) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TCR gamma/delta

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3 antibody(1).

References

Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Entrez Gene IDs

915 (Human); 12500 (Mouse); 25710 (Rat)

Alternate Names

delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCR gamma/delta; TCRG; TiT3; TRG