Mouse/Rat Integrin alpha 8 Antibody Summary
Phe38-Phe1007
Accession # A28ARA8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Mouse Integrin a8 antibody by Western Blot. Detection of Mouse Integrin a8 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Integrin_alpha_8_AF4076_Western_Blot_20029.jpg)
Detection of Mouse Integrin alpha 8 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Integrin a8 antibody in 4T1 Mouse Breast Cancer Cell Line by Immunocytochemistry (ICC). Integrin a8 antibody in 4T1 Mouse Breast Cancer Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/Integrin_alpha_8_AF4076_Immunocytochemistry__Immunofluorescence_17437.jpg)
Integrin alpha 8 in 4T1 Mouse Breast Cancer Cell Line. Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
![Detection of Mouse and Rat Integrin a8 antibody by Simple Western<sup>TM</sup>. Detection of Mouse and Rat Integrin a8 antibody by Simple Western<sup>TM</sup>.](https://resources.rndsystems.com/images/datasheets/antibody/Integrin_alpha_8_AF4076_Simple_Western_20113.jpg)
Detection of Mouse and Rat Integrin alpha 8 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
![Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence](https://resources.rndsystems.com/images/datasheets/af4076_mouse-rat-integrin-alpha-8-affinity-purified-polyclonal-ab-41202412401293.jpg)
Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence](https://resources.rndsystems.com/images/datasheets/af4076_mouse-rat-integrin-alpha-8-affinity-purified-polyclonal-ab-41202412401256.jpg)
Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.
![Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence](https://resources.rndsystems.com/images/datasheets/af4076_mouse-rat-integrin-alpha-8-affinity-purified-polyclonal-ab-41202412402547.jpg)
Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha 8 protein, respectively.
Product Datasheets
Citations for Mouse/Rat Integrin alpha 8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
24
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids
Authors: Huang B, Zeng Z, Li H et al.
bioRxiv
-
Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression
Authors: P Martino, R Sunkara, N Heitman, M Rangl, A Brown, N Saxena, L Grisanti, D Kohan, M Yanagisawa, M Rendl
Nature Cell Biology, 2023-01-30;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Chromatin accessibility and microRNA expression in nephron progenitor cells during kidney development
Authors: Andrew Clugston, Andrew Bodnar, Débora Malta Cerqueira, Yu Leng Phua, Alyssa Lawler, Kristy Boggs et al.
Genomics
-
Lef1 expression in fibroblasts maintains developmental potential in adult skin to regenerate wounds
Authors: Quan M Phan, Gracelyn M Fine, Lucia Salz, Gerardo G Herrera, Ben Wildman, Iwona M Driskell et al.
eLife
-
Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli
Authors: Sun-Sang J Sung
BIO-PROTOCOL
-
Molecular characterization of nephron progenitors and their early epithelial derivative structures in the nephrogenic zone of the canine fetal kidney
Authors: Rawah Faraj, Angela Irizarry-Alfonzo, Pawan Puri
European Journal of Histochemistry
-
Wnt11 directs nephron progenitor polarity and motile behavior ultimately determining nephron endowment
Authors: Lori L O'Brien, Alexander N Combes, Kieran M Short, Nils O Lindström, Peter H Whitney, Luise A Cullen-McEwen et al.
eLife
-
Aberrant DNA methylation of Tgfb1 in diabetic kidney mesangial cells
Authors: S Oba, N Ayuzawa, M Nishimoto, W Kawarazaki, K Ueda, D Hirohama, F Kawakami-M, T Shimosawa, T Marumo, T Fujita
Sci Rep, 2018-11-05;8(1):16338.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Hair follicle epidermal stem cells define a niche for tactile sensation
Authors: Chun-Chun Cheng, Ko Tsutsui, Toru Taguchi, Noriko Sanzen, Asako Nakagawa, Kisa Kakiguchi et al.
eLife
-
Lysyl oxidase like-2 contributes to renal fibrosis in Col4a3/Alport Mice.
Authors: Dominic Cosgrove, Brianna Dufek, Daniel T. Meehan, Duane Delimont, Michael Hartnett, Gina Samuelson et al.
Kidney International
-
Isolation of a unique hepatic stellate cell population expressing integrin alpha 8 from embryonic mouse livers
Authors: Tomohiro Ogawa, Yuchang Li, Ingrid Lua, Andrea Hartner, Kinji Asahina
Developmental Dynamics
-
Role of integrin alpha8 in murine model of lung fibrosis
Authors: CF Hung, CL Wilson, YH Chow, LM Schnapp
PLoS ONE, 2018-05-29;13(5):e0197937.
Species: Mouse
Sample Types: Protein, Whole Tissue
Applications: IHC, Western Blot -
Expression of the Alpha8 Integrin Chain Facilitates Phagocytosis by Renal Mesangial Cells
Authors: Ines Marek, Robert Becker, Fabian B. Fahlbusch, Carlos Menendez-Castro, Wolfgang Rascher, Christoph Daniel et al.
Cellular Physiology and Biochemistry
-
Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney
Authors: David A. D. Munro, Peter Hohenstein, Thomas M. Coate, Jamie A. Davies
Developmental Dynamics
-
Integrin alpha 8 Is Abundant in Human, Rat, and Mouse Trophoblasts
Authors: Sebastian Herdl, Hanna Huebner, Gudrun Volkert, Ines Marek, Carlos Menendez-Castro, Stephanie C. Noegel et al.
Reproductive Sciences
-
Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development
Authors: N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi
Dev. Cell, 2017-05-22;41(4):349-365.e3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development
Authors: K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt
Am. J. Pathol., 2017-05-17;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
J. Immunol, 2017-02-20;0(0):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage
PLoS ONE, 2016-12-13;11(12):e0168343.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC -
Laminin alpha2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis.
Authors: Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, Cosgrove D
PLoS ONE, 2014-06-10;9(6):e99083.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
Nature, 2013-12-12;504(7479):277-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins.
J. Cell Biol., 2012-05-21;197(5):677-89.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Endothelin A receptor activation on mesangial cells initiates Alport glomerular disease.
Authors: Dufek Brianna, Meehan Daniel T, Delimont Duane et al.
Kidney International
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse/Rat Integrin alpha 8 Antibody
Average Rating: 5 (Based on 3 Reviews)
Have you used Mouse/Rat Integrin alpha 8 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Stables HEK293 expressing ITGA8 were lysed in RIPA and used for western blot analysis. Blot was incubated overnight with 5% milk blocking solution followed by an overnight incubation with 0.4ug/ml of goat anti-ITGA8 in 5% milk. Blot was incubated the next day with 1:3,000 anti-goat HRP secondary antibody and developed employing a film processor. 293 ITGA8: Stable cell line. 293: untransfected cells. Actin was used as loading control.