Mouse R-Spondin 1 Antibody

Catalog #: MAB3474 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3474
MAB3474-SP

beta -Catenin Response Induced by R-Spondin 1 and Neutralization by Mouse R‑Spondin 1 Antibody.

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Mouse R-Spondin 1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse (rm) R‑Spondin 1 in ELISA. In ELISA, no cross-reactivity with rmR-Spondin 3 and rhR-Spondin 1 is observed.

Source

Monoclonal Rat IgG_2B Clone # 852010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse R‑Spondin 1
Met1-Gln265
Accession # Q9Z132

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize R‑Spondin 1-induced beta -catenin response in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2 μg/mL in the presence of 500 ng/mL Recombinant Mouse R‑Spondin 1 and 5 ng/mL Recombinant Mouse Wnt-3a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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beta -Catenin Response Induced by R-Spondin 1 and Neutralization by Mouse R‑Spondin 1 Antibody. Recombinant Mouse R-Spondin 1 (7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R-Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R-Spondin 1 Mono-clonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (1324-WN). The ND₅₀ is typically 0.4-2 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 1

RSPO-1 (roof plate-specific spondin; also R-Spondin 1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-Spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.

Long Name

Roof Plate-specific Spondin 1

Entrez Gene IDs

284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)

Alternate Names

Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1